Analysis of Bisulfite Via a Nitro Derivative of Cyanine-3 (NCy3) in the Microfluidic Channel

NCy3 , a derivative of Cyanine 3 with a nitro substituent, showed a high reactivity to bisulfite in aqueous media, instantly leading to ratiometric change of absorption spectra and significant fluorescence quenching. Applied in the microfluidic channel, NCy3 functionalize as a sensitive approach for...

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Veröffentlicht in:	Journal of fluorescence 2019-05, Vol.29 (3), p.523-529
Hauptverfasser:	Houtwed, Haley A., Xie, Meng, Ahmad, Aatiya, Masters, Cody D., Davison, Melissa M., Kounovsky-Shafer, Kristy, Cao, Haishi
Format:	Artikel
Sprache:	eng
Schlagworte:	Absorption spectra Analytical Chemistry Aqueous solutions Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Fluorescence Microfluidics Short Communication
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	NCy3 , a derivative of Cyanine 3 with a nitro substituent, showed a high reactivity to bisulfite in aqueous media, instantly leading to ratiometric change of absorption spectra and significant fluorescence quenching. Applied in the microfluidic channel, NCy3 functionalize as a sensitive approach for quantitative detection of bisulfite, particularly for samples with a small volume.
ISSN:	1053-0509 1573-4994 1573-4994
DOI:	10.1007/s10895-019-02394-0