Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins

A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotav...

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Veröffentlicht in:	Microbiology resource announcements 2019-07, Vol.8 (27)
Hauptverfasser:	Philip, Asha A, Herrin, Brittany E, Garcia, Maximiliano L, Abad, Andrew T, Katen, Sarah P, Patton, John T
Format:	Artikel
Sprache:	eng
Schlagworte:	Culture Collections/Mutant Libraries
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.
ISSN:	2576-098X 2576-098X
DOI:	10.1128/mra.00523-19