Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability

Effective methods to detect viable , the main causative agent of tuberculosis (TB), are urgently needed. To date, cultivation of is the gold standard, which depends on initial sample processing with -acetyl-l-cysteine-sodium hydroxide (NALC-NaOH), chemicals that compromise viability and, consequentl...

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Veröffentlicht in:Journal of clinical microbiology 2019-07, Vol.57 (7)
Hauptverfasser: Mtafya, Bariki, Sabiiti, Wilber, Sabi, Issa, John, Joseph, Sichone, Emanuel, Ntinginya, Nyanda E, Gillespie, Stephen H
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Sprache:eng
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Zusammenfassung:Effective methods to detect viable , the main causative agent of tuberculosis (TB), are urgently needed. To date, cultivation of is the gold standard, which depends on initial sample processing with -acetyl-l-cysteine-sodium hydroxide (NALC-NaOH), chemicals that compromise viability and, consequently, the performance of downstream tests. We applied culture and the novel molecular bacterial load assay (MBLA) to measure the loss of viability following NALC-NaOH treatment of H37Rv pure culture and clinical sputum samples from pulmonary TB patients. Compared to the bacterial loads of untreated controls, NALC-NaOH treatment of reduced the MBLA-detectable bacillary load (estimated number of CFU [eCFU] per milliliter) by 0.66 ± 0.21 log at 23°C (  = 0.018) and 0.72 ± 0.08 log at 30°C (  = 0.013). Likewise, NALC-NaOH treatment reduced the viable count on solid culture by 0.84 ± 0.02 log CFU/ml at 23°C (  
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01992-18