Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Effective methods to detect viable , the main causative agent of tuberculosis (TB), are urgently needed. To date, cultivation of is the gold standard, which depends on initial sample processing with -acetyl-l-cysteine-sodium hydroxide (NALC-NaOH), chemicals that compromise viability and, consequentl...
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Veröffentlicht in: | Journal of clinical microbiology 2019-07, Vol.57 (7) |
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Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Effective methods to detect viable
, the main causative agent of tuberculosis (TB), are urgently needed. To date, cultivation of
is the gold standard, which depends on initial sample processing with
-acetyl-l-cysteine-sodium hydroxide (NALC-NaOH), chemicals that compromise
viability and, consequently, the performance of downstream tests. We applied culture and the novel molecular bacterial load assay (MBLA) to measure the loss of
viability following NALC-NaOH treatment of
H37Rv pure culture and clinical sputum samples from pulmonary TB patients. Compared to the bacterial loads of untreated controls, NALC-NaOH treatment of
reduced the MBLA-detectable bacillary load (estimated number of CFU [eCFU] per milliliter) by 0.66 ± 0.21 log
at 23°C (
= 0.018) and 0.72 ± 0.08 log
at 30°C (
= 0.013). Likewise, NALC-NaOH treatment reduced the viable count on solid culture by 0.84 ± 0.02 log
CFU/ml at 23°C (
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ISSN: | 0095-1137 1098-660X |
DOI: | 10.1128/JCM.01992-18 |