Luteinizing Hormone-Releasing Hormone (LHRH) Neurons Maintained in Hypothalamic Slice Explant Cultures Exhibit a Rapid LHRH mRNA Turnover Rate
Evidence indicates that neuropeptide gene expression is tightly coupled to biosynthesis and secretion. Moreover, rhythmic gene expression often accompanies rhythmic secretion. Luteinizing hormone-releasing hormone (LHRH) neurosecretion, which regulates gonadal function, is pulsatile, with interpulse...
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| Veröffentlicht in: | The Journal of neuroscience 1997-12, Vol.17 (24), p.9481-9491 |
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| creator | Maurer, Jennifer A Wray, Susan |
| description | Evidence indicates that neuropeptide gene expression is tightly coupled to biosynthesis and secretion. Moreover, rhythmic gene expression often accompanies rhythmic secretion. Luteinizing hormone-releasing hormone (LHRH) neurosecretion, which regulates gonadal function, is pulsatile, with interpulse intervals of approximately 1 hr and pulse decays of |
| doi_str_mv | 10.1523/jneurosci.17-24-09481.1997 |
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Moreover, rhythmic gene expression often accompanies rhythmic secretion. Luteinizing hormone-releasing hormone (LHRH) neurosecretion, which regulates gonadal function, is pulsatile, with interpulse intervals of approximately 1 hr and pulse decays of <30 min in rats. As a basis for a rapid fall in peptide secretion, we hypothesize that LHRH mRNA levels rapidly decay. To address this hypothesis, we examined LHRH mRNA turnover in primary postnatal LHRH neurons maintained in long-term hypothalamic/preoptic area slice explant cultures, using in situ hybridization histochemistry (ISHH). Relative LHRH mRNA content per cell was quantitated by single-cell analysis after transcription inhibition with 5, 6-dichloro-1-D-ribofuranosyl-benzimidazole (DRB) or actinomycin D. Cultures were maintained in serum-free medium with tetrodotoxin to suppress spontaneous electrical activity and hence assess only intrinsic cellular activity. A plot of LHRH mRNA level per cell versus DRB treatment time showed a rapid initial decay of LHRH mRNA (t1/2, 5-13 min), followed by a slower decay rate (t1/2, 329-344 hr). LHRH cell number after drug treatment as determined by immunocytochemistry did not change. Comparison of mammalian LHRH mRNA 3'-untranslated regions showed two conserved regions. These data indicate that, in primary LHRH neurons, LHRH mRNA has an intrinsically high rate of turnover and a mRNA stabilization component. Foremost, decay of LHRH mRNA, the fastest reported for a neuropeptide to date, corresponds to the decay of LHRH peptide pulses.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/jneurosci.17-24-09481.1997</identifier><identifier>PMID: 9391004</identifier><language>eng</language><publisher>United States: Soc Neuroscience</publisher><subject>Animals ; Cell Culture Techniques - methods ; Dichlororibofuranosylbenzimidazole ; Gene Expression - drug effects ; Gene Expression - physiology ; Gonadotropin-Releasing Hormone - analysis ; Gonadotropin-Releasing Hormone - genetics ; Hippocampus - cytology ; Neurons - chemistry ; Neurons - cytology ; Neurons - physiology ; Nucleic Acid Synthesis Inhibitors ; Preoptic Area - cytology ; Rats ; RNA, Messenger - metabolism</subject><ispartof>The Journal of neuroscience, 1997-12, Vol.17 (24), p.9481-9491</ispartof><rights>Copyright © 1997 Society for Neuroscience 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-ab2672e79c5b13d2ca0f5d74b2608e2af4ae53588afc398436d3e3ee0ccfe8613</citedby><cites>FETCH-LOGICAL-c484t-ab2672e79c5b13d2ca0f5d74b2608e2af4ae53588afc398436d3e3ee0ccfe8613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573397/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573397/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9391004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maurer, Jennifer A</creatorcontrib><creatorcontrib>Wray, Susan</creatorcontrib><title>Luteinizing Hormone-Releasing Hormone (LHRH) Neurons Maintained in Hypothalamic Slice Explant Cultures Exhibit a Rapid LHRH mRNA Turnover Rate</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Evidence indicates that neuropeptide gene expression is tightly coupled to biosynthesis and secretion. Moreover, rhythmic gene expression often accompanies rhythmic secretion. Luteinizing hormone-releasing hormone (LHRH) neurosecretion, which regulates gonadal function, is pulsatile, with interpulse intervals of approximately 1 hr and pulse decays of <30 min in rats. As a basis for a rapid fall in peptide secretion, we hypothesize that LHRH mRNA levels rapidly decay. To address this hypothesis, we examined LHRH mRNA turnover in primary postnatal LHRH neurons maintained in long-term hypothalamic/preoptic area slice explant cultures, using in situ hybridization histochemistry (ISHH). Relative LHRH mRNA content per cell was quantitated by single-cell analysis after transcription inhibition with 5, 6-dichloro-1-D-ribofuranosyl-benzimidazole (DRB) or actinomycin D. Cultures were maintained in serum-free medium with tetrodotoxin to suppress spontaneous electrical activity and hence assess only intrinsic cellular activity. A plot of LHRH mRNA level per cell versus DRB treatment time showed a rapid initial decay of LHRH mRNA (t1/2, 5-13 min), followed by a slower decay rate (t1/2, 329-344 hr). LHRH cell number after drug treatment as determined by immunocytochemistry did not change. Comparison of mammalian LHRH mRNA 3'-untranslated regions showed two conserved regions. These data indicate that, in primary LHRH neurons, LHRH mRNA has an intrinsically high rate of turnover and a mRNA stabilization component. Foremost, decay of LHRH mRNA, the fastest reported for a neuropeptide to date, corresponds to the decay of LHRH peptide pulses.</description><subject>Animals</subject><subject>Cell Culture Techniques - methods</subject><subject>Dichlororibofuranosylbenzimidazole</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - physiology</subject><subject>Gonadotropin-Releasing Hormone - analysis</subject><subject>Gonadotropin-Releasing Hormone - genetics</subject><subject>Hippocampus - cytology</subject><subject>Neurons - chemistry</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Nucleic Acid Synthesis Inhibitors</subject><subject>Preoptic Area - cytology</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9v0zAUxSMEGmXwEZAsHvjzkGLHTpzwgDRVhQyVTsq2Z8t1blpPjhPsZN32IfjMOLSaxhMPlqVzjn--VyeK3hE8J2lCP99YGF3nlZ4THicsxgXLyZwUBX8WzUKiCCImz6MZTjiOM8bZy-iV9zcYY44JP4lOCloQjNks-r0aB9BWP2i7RWXn2s5CXIEB6Z8o6OOqrMpPaD39az36KbUdwoEaaYvK-74bdtLIVit0abQCtLzrjbQDWoxmGB34IOz0Rg9Iokr2ukYTD7XV-gxdjc52t-CCMcDr6EUjjYc3x_s0uv62vFqU8eri-_nibBUrlrMhlpsk4wnwQqUbQutESdykNWdBxjkksmESUprmuWwULXJGs5oCBcBKNZBnhJ5GXw_cfty0UCuwg5NG9E630t2LTmrxr2P1Tmy7W5GlnNKCB8D7I8B1v0bwg2i1V2DC1tCNXvCCpUnK8H-DJKNZkrFppC-HoArVegfN4zQEi6l28WO9vK4uLhfngnCRMPG3djHVHh6_fbrP49Njz8H_cPB3ervbawfCt9KYkCZiv98feBOO_gH4druP</recordid><startdate>19971215</startdate><enddate>19971215</enddate><creator>Maurer, Jennifer A</creator><creator>Wray, Susan</creator><general>Soc Neuroscience</general><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19971215</creationdate><title>Luteinizing Hormone-Releasing Hormone (LHRH) Neurons Maintained in Hypothalamic Slice Explant Cultures Exhibit a Rapid LHRH mRNA Turnover Rate</title><author>Maurer, Jennifer A ; Wray, Susan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-ab2672e79c5b13d2ca0f5d74b2608e2af4ae53588afc398436d3e3ee0ccfe8613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Cell Culture Techniques - methods</topic><topic>Dichlororibofuranosylbenzimidazole</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - physiology</topic><topic>Gonadotropin-Releasing Hormone - analysis</topic><topic>Gonadotropin-Releasing Hormone - genetics</topic><topic>Hippocampus - cytology</topic><topic>Neurons - chemistry</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>Nucleic Acid Synthesis Inhibitors</topic><topic>Preoptic Area - cytology</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maurer, Jennifer A</creatorcontrib><creatorcontrib>Wray, Susan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maurer, Jennifer A</au><au>Wray, Susan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Luteinizing Hormone-Releasing Hormone (LHRH) Neurons Maintained in Hypothalamic Slice Explant Cultures Exhibit a Rapid LHRH mRNA Turnover Rate</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>1997-12-15</date><risdate>1997</risdate><volume>17</volume><issue>24</issue><spage>9481</spage><epage>9491</epage><pages>9481-9491</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Evidence indicates that neuropeptide gene expression is tightly coupled to biosynthesis and secretion. Moreover, rhythmic gene expression often accompanies rhythmic secretion. Luteinizing hormone-releasing hormone (LHRH) neurosecretion, which regulates gonadal function, is pulsatile, with interpulse intervals of approximately 1 hr and pulse decays of <30 min in rats. As a basis for a rapid fall in peptide secretion, we hypothesize that LHRH mRNA levels rapidly decay. To address this hypothesis, we examined LHRH mRNA turnover in primary postnatal LHRH neurons maintained in long-term hypothalamic/preoptic area slice explant cultures, using in situ hybridization histochemistry (ISHH). Relative LHRH mRNA content per cell was quantitated by single-cell analysis after transcription inhibition with 5, 6-dichloro-1-D-ribofuranosyl-benzimidazole (DRB) or actinomycin D. Cultures were maintained in serum-free medium with tetrodotoxin to suppress spontaneous electrical activity and hence assess only intrinsic cellular activity. A plot of LHRH mRNA level per cell versus DRB treatment time showed a rapid initial decay of LHRH mRNA (t1/2, 5-13 min), followed by a slower decay rate (t1/2, 329-344 hr). LHRH cell number after drug treatment as determined by immunocytochemistry did not change. Comparison of mammalian LHRH mRNA 3'-untranslated regions showed two conserved regions. These data indicate that, in primary LHRH neurons, LHRH mRNA has an intrinsically high rate of turnover and a mRNA stabilization component. Foremost, decay of LHRH mRNA, the fastest reported for a neuropeptide to date, corresponds to the decay of LHRH peptide pulses.</abstract><cop>United States</cop><pub>Soc Neuroscience</pub><pmid>9391004</pmid><doi>10.1523/jneurosci.17-24-09481.1997</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Culture Techniques - methods Dichlororibofuranosylbenzimidazole Gene Expression - drug effects Gene Expression - physiology Gonadotropin-Releasing Hormone - analysis Gonadotropin-Releasing Hormone - genetics Hippocampus - cytology Neurons - chemistry Neurons - cytology Neurons - physiology Nucleic Acid Synthesis Inhibitors Preoptic Area - cytology Rats RNA, Messenger - metabolism |
| title | Luteinizing Hormone-Releasing Hormone (LHRH) Neurons Maintained in Hypothalamic Slice Explant Cultures Exhibit a Rapid LHRH mRNA Turnover Rate |
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