Characterization of proopiomelanocortin mRNA detected by in situ hybridization

In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe. We have characterized in situ hybridization in rat pituitary using a cDNA probe directed against a portion of proopiomelanocortin (POMC) mRNA to show its specificity in...

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Veröffentlicht in:	The Journal of neuroscience 1986-01, Vol.6 (1), p.38-42
Hauptverfasser:	Kelsey, JE, Watson, SJ, Burke, S, Akil, H, Roberts, JL
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences DNA - analysis Fundamental and applied biological sciences. Psychology Haloperidol - pharmacology Male MRNA Nucleic Acid Hybridization Nucleic acids pituitary Pituitary Gland - analysis Pituitary Gland - drug effects pro-opiomelanocortin Pro-Opiomelanocortin - genetics Rats Rats, Inbred Strains RNA, Messenger - analysis Rna, ribonucleoproteins
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Beschreibung
Zusammenfassung:	In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe. We have characterized in situ hybridization in rat pituitary using a cDNA probe directed against a portion of proopiomelanocortin (POMC) mRNA to show its specificity in tissue sections. This characterization includes the size of the target mRNA from tissue processed for in situ hybridization, the dissociation pattern of the cDNA:mRNA hybrid, and quantitation of changes in POMC mRNA levels in the rat pituitary intermediate lobe detected both by in situ hybridization and "dot blot" techniques.
ISSN:	0270-6474 1529-2401
DOI:	10.1523/jneurosci.06-01-00038.1986