Fast, in vivo voltage imaging using a red fluorescent indicator

Genetically encoded voltage indicators (GEVIs) are emerging optical tools for acquiring brain-wide cell-type-specific functional data at unparalleled temporal resolution. To broaden the application of GEVIs in high-speed multispectral imaging, we used a high-throughput strategy to develop voltage-ac...

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Veröffentlicht in:	Nature methods 2018-12, Vol.15 (12), p.1108-1116
Hauptverfasser:	Kannan, Madhuvanthi, Vasan, Ganesh, Huang, Cheng, Haziza, Simon, Li, Jin Zhong, Inan, Hakan, Schnitzer, Mark J., Pieribone, Vincent A.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1888/2249 631/1647/245/2225 631/1647/334/1582/715 631/1647/334/1874/345 631/378/87 Action Potentials Animal behavior Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Brain Brain - cytology Brain - physiology Brain slice preparation Cells, Cultured Coding Cortex Data acquisition Data processing Drosophila Drosophila melanogaster - metabolism Electric potential Electrophysiological Phenomena Electrophysiology Female Fluorescent Dyes - chemistry Fluorescent indicators Fruit flies Genetic code HEK293 Cells High speed Hippocampus Humans Image Processing, Computer-Assisted - methods Imaging Insects Life Sciences Luminescent Proteins - metabolism Male Mice Mice, Inbred C57BL Microscopy, Fluorescence - methods Neuroimaging Neurons Neurons - cytology Neurons - physiology Optogenetics Physiological aspects Physiology Proteomics Red Fluorescent Protein Red fluorescent proteins Sensitivity enhancement Temporal resolution Voltage Voltage indicators
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Beschreibung
Zusammenfassung:	Genetically encoded voltage indicators (GEVIs) are emerging optical tools for acquiring brain-wide cell-type-specific functional data at unparalleled temporal resolution. To broaden the application of GEVIs in high-speed multispectral imaging, we used a high-throughput strategy to develop voltage-activated red neuronal activity monitor (VARNAM), a fusion of the fast Acetabularia opsin and the bright red fluorophore mRuby3. Imageable under the modest illumination intensities required by bright green probes (
ISSN:	1548-7091 1548-7105
DOI:	10.1038/s41592-018-0188-7