A multicenter comparison of MOG-IgG cell-based assays
OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers. METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis...
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| Veröffentlicht in: | Neurology 2019-03, Vol.92 (11), p.e1250-e1255 |
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| container_title | Neurology |
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| creator | Waters, Patrick J Komorowski, Lars Woodhall, Mark Lederer, Sabine Majed, Masoud Fryer, Jim Mills, John Flanagan, Eoin P Irani, Sarosh R Kunchok, Amy C McKeon, Andrew Pittock, Sean J |
| description | OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.
METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay).
RESULTSOf 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as followsclinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone.
CONCLUSIONSOverall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders. |
| doi_str_mv | 10.1212/WNL.0000000000007096 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6511109</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2190487655</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4576-79cf9ac057091380b04aee0c0b47071567c86b15698360dc6e396666949cf2a93</originalsourceid><addsrcrecordid>eNp9kM1OAjEUhRujEUTfwJhZuhm8nZn-bUwIUSRB2Wh013RKgdEZii0j4e3tBCTowru5i3vO6emH0CWGLk5wcvP6NOrCwTAQ9Ai1MUloTNPk7Ri1ARIep5zxFjrz_h0gHJk4Ra0UWMJTIG1EelFVl6tCm8XKuEjbaqlc4e0istPocTyIh7NBpE1ZxrnyZhIp79XGn6OTqSq9udjtDnq5v3vuP8Sj8WDY741inRFGYyb0VCgNJHTDKYccMmUMaMgzBgwTyjSnediCpxQmmppU0DAiC8ZEibSDbre5yzqvzKQp6VQpl66olNtIqwr5-7Io5nJmvyQlGGNoAq53Ac5-1savZFX45jtqYWztZYIFZJxRQoI020q1s947M90_g0E2xGUgLv8SD7arw4p70w_iIOBbwdqWAbH_KOu1cXJuVLma_5_9DfCCi3s</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2190487655</pqid></control><display><type>article</type><title>A multicenter comparison of MOG-IgG cell-based assays</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><source>Alma/SFX Local Collection</source><creator>Waters, Patrick J ; Komorowski, Lars ; Woodhall, Mark ; Lederer, Sabine ; Majed, Masoud ; Fryer, Jim ; Mills, John ; Flanagan, Eoin P ; Irani, Sarosh R ; Kunchok, Amy C ; McKeon, Andrew ; Pittock, Sean J</creator><creatorcontrib>Waters, Patrick J ; Komorowski, Lars ; Woodhall, Mark ; Lederer, Sabine ; Majed, Masoud ; Fryer, Jim ; Mills, John ; Flanagan, Eoin P ; Irani, Sarosh R ; Kunchok, Amy C ; McKeon, Andrew ; Pittock, Sean J</creatorcontrib><description>OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.
METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay).
RESULTSOf 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as followsclinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone.
CONCLUSIONSOverall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders.</description><identifier>ISSN: 0028-3878</identifier><identifier>EISSN: 1526-632X</identifier><identifier>DOI: 10.1212/WNL.0000000000007096</identifier><identifier>PMID: 30728305</identifier><language>eng</language><publisher>United States: American Academy of Neurology</publisher><subject>Autoantibodies - analysis ; Autoantibodies - immunology ; Case-Control Studies ; Encephalomyelitis, Acute Disseminated - diagnosis ; Encephalomyelitis, Acute Disseminated - immunology ; Flow Cytometry ; HEK293 Cells ; Humans ; Hypergammaglobulinemia ; Immunoglobulin G - analysis ; Immunoglobulin G - immunology ; Microscopy, Fluorescence ; Multiple Sclerosis ; Myelin-Oligodendrocyte Glycoprotein - genetics ; Myelin-Oligodendrocyte Glycoprotein - immunology ; Myelitis, Transverse ; Neuromyelitis Optica - diagnosis ; Neuromyelitis Optica - immunology ; Optic Neuritis - diagnosis ; Optic Neuritis - immunology ; Serologic Tests - methods ; Transfection</subject><ispartof>Neurology, 2019-03, Vol.92 (11), p.e1250-e1255</ispartof><rights>2019 American Academy of Neurology</rights><rights>2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.</rights><rights>2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology. 2019 2019 American Academy of Neurology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4576-79cf9ac057091380b04aee0c0b47071567c86b15698360dc6e396666949cf2a93</citedby><cites>FETCH-LOGICAL-c4576-79cf9ac057091380b04aee0c0b47071567c86b15698360dc6e396666949cf2a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30728305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Waters, Patrick J</creatorcontrib><creatorcontrib>Komorowski, Lars</creatorcontrib><creatorcontrib>Woodhall, Mark</creatorcontrib><creatorcontrib>Lederer, Sabine</creatorcontrib><creatorcontrib>Majed, Masoud</creatorcontrib><creatorcontrib>Fryer, Jim</creatorcontrib><creatorcontrib>Mills, John</creatorcontrib><creatorcontrib>Flanagan, Eoin P</creatorcontrib><creatorcontrib>Irani, Sarosh R</creatorcontrib><creatorcontrib>Kunchok, Amy C</creatorcontrib><creatorcontrib>McKeon, Andrew</creatorcontrib><creatorcontrib>Pittock, Sean J</creatorcontrib><title>A multicenter comparison of MOG-IgG cell-based assays</title><title>Neurology</title><addtitle>Neurology</addtitle><description>OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.
METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay).
RESULTSOf 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as followsclinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone.
CONCLUSIONSOverall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders.</description><subject>Autoantibodies - analysis</subject><subject>Autoantibodies - immunology</subject><subject>Case-Control Studies</subject><subject>Encephalomyelitis, Acute Disseminated - diagnosis</subject><subject>Encephalomyelitis, Acute Disseminated - immunology</subject><subject>Flow Cytometry</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Hypergammaglobulinemia</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin G - immunology</subject><subject>Microscopy, Fluorescence</subject><subject>Multiple Sclerosis</subject><subject>Myelin-Oligodendrocyte Glycoprotein - genetics</subject><subject>Myelin-Oligodendrocyte Glycoprotein - immunology</subject><subject>Myelitis, Transverse</subject><subject>Neuromyelitis Optica - diagnosis</subject><subject>Neuromyelitis Optica - immunology</subject><subject>Optic Neuritis - diagnosis</subject><subject>Optic Neuritis - immunology</subject><subject>Serologic Tests - methods</subject><subject>Transfection</subject><issn>0028-3878</issn><issn>1526-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1OAjEUhRujEUTfwJhZuhm8nZn-bUwIUSRB2Wh013RKgdEZii0j4e3tBCTowru5i3vO6emH0CWGLk5wcvP6NOrCwTAQ9Ai1MUloTNPk7Ri1ARIep5zxFjrz_h0gHJk4Ra0UWMJTIG1EelFVl6tCm8XKuEjbaqlc4e0istPocTyIh7NBpE1ZxrnyZhIp79XGn6OTqSq9udjtDnq5v3vuP8Sj8WDY741inRFGYyb0VCgNJHTDKYccMmUMaMgzBgwTyjSnediCpxQmmppU0DAiC8ZEibSDbre5yzqvzKQp6VQpl66olNtIqwr5-7Io5nJmvyQlGGNoAq53Ac5-1savZFX45jtqYWztZYIFZJxRQoI020q1s947M90_g0E2xGUgLv8SD7arw4p70w_iIOBbwdqWAbH_KOu1cXJuVLma_5_9DfCCi3s</recordid><startdate>20190312</startdate><enddate>20190312</enddate><creator>Waters, Patrick J</creator><creator>Komorowski, Lars</creator><creator>Woodhall, Mark</creator><creator>Lederer, Sabine</creator><creator>Majed, Masoud</creator><creator>Fryer, Jim</creator><creator>Mills, John</creator><creator>Flanagan, Eoin P</creator><creator>Irani, Sarosh R</creator><creator>Kunchok, Amy C</creator><creator>McKeon, Andrew</creator><creator>Pittock, Sean J</creator><general>American Academy of Neurology</general><general>Lippincott Williams & Wilkins</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190312</creationdate><title>A multicenter comparison of MOG-IgG cell-based assays</title><author>Waters, Patrick J ; Komorowski, Lars ; Woodhall, Mark ; Lederer, Sabine ; Majed, Masoud ; Fryer, Jim ; Mills, John ; Flanagan, Eoin P ; Irani, Sarosh R ; Kunchok, Amy C ; McKeon, Andrew ; Pittock, Sean J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4576-79cf9ac057091380b04aee0c0b47071567c86b15698360dc6e396666949cf2a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Autoantibodies - analysis</topic><topic>Autoantibodies - immunology</topic><topic>Case-Control Studies</topic><topic>Encephalomyelitis, Acute Disseminated - diagnosis</topic><topic>Encephalomyelitis, Acute Disseminated - immunology</topic><topic>Flow Cytometry</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Hypergammaglobulinemia</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin G - immunology</topic><topic>Microscopy, Fluorescence</topic><topic>Multiple Sclerosis</topic><topic>Myelin-Oligodendrocyte Glycoprotein - genetics</topic><topic>Myelin-Oligodendrocyte Glycoprotein - immunology</topic><topic>Myelitis, Transverse</topic><topic>Neuromyelitis Optica - diagnosis</topic><topic>Neuromyelitis Optica - immunology</topic><topic>Optic Neuritis - diagnosis</topic><topic>Optic Neuritis - immunology</topic><topic>Serologic Tests - methods</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Waters, Patrick J</creatorcontrib><creatorcontrib>Komorowski, Lars</creatorcontrib><creatorcontrib>Woodhall, Mark</creatorcontrib><creatorcontrib>Lederer, Sabine</creatorcontrib><creatorcontrib>Majed, Masoud</creatorcontrib><creatorcontrib>Fryer, Jim</creatorcontrib><creatorcontrib>Mills, John</creatorcontrib><creatorcontrib>Flanagan, Eoin P</creatorcontrib><creatorcontrib>Irani, Sarosh R</creatorcontrib><creatorcontrib>Kunchok, Amy C</creatorcontrib><creatorcontrib>McKeon, Andrew</creatorcontrib><creatorcontrib>Pittock, Sean J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Waters, Patrick J</au><au>Komorowski, Lars</au><au>Woodhall, Mark</au><au>Lederer, Sabine</au><au>Majed, Masoud</au><au>Fryer, Jim</au><au>Mills, John</au><au>Flanagan, Eoin P</au><au>Irani, Sarosh R</au><au>Kunchok, Amy C</au><au>McKeon, Andrew</au><au>Pittock, Sean J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A multicenter comparison of MOG-IgG cell-based assays</atitle><jtitle>Neurology</jtitle><addtitle>Neurology</addtitle><date>2019-03-12</date><risdate>2019</risdate><volume>92</volume><issue>11</issue><spage>e1250</spage><epage>e1255</epage><pages>e1250-e1255</pages><issn>0028-3878</issn><eissn>1526-632X</eissn><abstract>OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.
METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay).
RESULTSOf 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as followsclinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone.
CONCLUSIONSOverall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders.</abstract><cop>United States</cop><pub>American Academy of Neurology</pub><pmid>30728305</pmid><doi>10.1212/WNL.0000000000007096</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Autoantibodies - analysis Autoantibodies - immunology Case-Control Studies Encephalomyelitis, Acute Disseminated - diagnosis Encephalomyelitis, Acute Disseminated - immunology Flow Cytometry HEK293 Cells Humans Hypergammaglobulinemia Immunoglobulin G - analysis Immunoglobulin G - immunology Microscopy, Fluorescence Multiple Sclerosis Myelin-Oligodendrocyte Glycoprotein - genetics Myelin-Oligodendrocyte Glycoprotein - immunology Myelitis, Transverse Neuromyelitis Optica - diagnosis Neuromyelitis Optica - immunology Optic Neuritis - diagnosis Optic Neuritis - immunology Serologic Tests - methods Transfection |
| title | A multicenter comparison of MOG-IgG cell-based assays |
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