A multicenter comparison of MOG-IgG cell-based assays

OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers. METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis...

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Veröffentlicht in:Neurology 2019-03, Vol.92 (11), p.e1250-e1255
Hauptverfasser: Waters, Patrick J, Komorowski, Lars, Woodhall, Mark, Lederer, Sabine, Majed, Masoud, Fryer, Jim, Mills, John, Flanagan, Eoin P, Irani, Sarosh R, Kunchok, Amy C, McKeon, Andrew, Pittock, Sean J
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Sprache:eng
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Zusammenfassung:OBJECTIVESTo compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers. METHODSSerum samples from 394 patients were as followsacute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay). RESULTSOf 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as followsclinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone. CONCLUSIONSOverall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders.
ISSN:0028-3878
1526-632X
DOI:10.1212/WNL.0000000000007096