Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adult neural stem cells. •High-throughput clonal analysis shows a common origin of ependymal and B1 stem cells•Ependymal cells are mostly produced through symmetric divisions•B1 cells are generated through asymmetric divisions with ependymal cells•Geminin family members tune the proportion of ependymal and B1 stem cells in clones Adult neurogenesis persists in mammals. Ortiz-Álvarez et al. analyze the clonal relationship among ependymal cells and B1 astrocytes, which together compose the adult neurogenic niche. They show that their proportion is tuned by the DNA replication regulators Geminin and GemC1.