In vitro neuronal network activity as a new functional diagnostic system to detect effects of Cerebrospinal fluid from autoimmune encephalitis patients

The intent of this study was to investigate if cerebrospinal fluid (CSF) from autoimmune encephalitis (AE) patients regulates in vitro neuronal network activity differentially to healthy human control CSF (hCSF). To this end, electrophysiological effects of CSF from AE patients or hCSF were measured...

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Veröffentlicht in:	Scientific reports 2019-04, Vol.9 (1), p.5591-5591, Article 5591
Hauptverfasser:	Koch, Henner, Niturad, Cristina E., Theiss, Stephan, Bien, Christian G., Elger, Christian, Wandinger, Klaus-Peter, Vincent, Angela, Malter, Michael, Körtvelyessy, Peter, Lerche, Holger, Dihné, Marcel
Format:	Artikel
Sprache:	eng
Schlagworte:	13/106 2-Amino-5-phosphonovalerate - pharmacology 631/378/371 64/60 692/53 Animals Anti-N-Methyl-D-Aspartate Receptor Encephalitis - diagnosis Anti-N-Methyl-D-Aspartate Receptor Encephalitis - drug therapy Autoimmune diseases Cerebrospinal fluid Cerebrospinal Fluid - drug effects Cerebrospinal Fluid - physiology Diagnostic tests Encephalitis Encephalitis - diagnosis Encephalitis - drug therapy Encephalitis - metabolism Encephalitis - pathology Female Firing pattern Firing rate Glioma Glutamate receptors Hashimoto Disease - diagnosis Hashimoto Disease - drug therapy Hashimoto Disease - metabolism Hashimoto Disease - pathology Humanities and Social Sciences Humans Leucine LGI1 protein Male Mice Middle Aged multidisciplinary N-Methyl-D-aspartic acid receptors Nervous System - drug effects Nervous System - metabolism Nervous System - pathology Neural networks Neurons - drug effects Neurons - metabolism Neurons - pathology Pregnancy Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors Science Science (multidisciplinary)
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Zusammenfassung:	The intent of this study was to investigate if cerebrospinal fluid (CSF) from autoimmune encephalitis (AE) patients regulates in vitro neuronal network activity differentially to healthy human control CSF (hCSF). To this end, electrophysiological effects of CSF from AE patients or hCSF were measured by in vitro neuronal network activity (ivNNA) recorded with microelectrode arrays (MEA). CSF from patients with either N-methyl-D-aspartate-receptor-antibody (pCSF NMDAR , n = 7) or Leucine-rich-glioma-inactivated-1-Ab (pCSF LGI1 , n = 6) associated AE suppressed global spiking activity of neuronal networks by a factor of 2.17 ( p
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-019-41849-z