High performance image mapping spectrometer (IMS) for snapshot hyperspectral imaging applications

A high performance, snapshot Image Mapping Spectrometer was developed that provides fast image acquisition (100 Hz) of 16 bit hyperspectral data cubes (210x210x46) over a spectral range of 515-842 nm. Essential details of the opto-mechanical design are presented. Spectral accuracy, precision, and im...

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Veröffentlicht in:Optics express 2019-01, Vol.27 (2), p.1597-1612
Hauptverfasser: Pawlowski, Michal E, Dwight, Jason G, Nguyen, Thuc-Uyen, Tkaczyk, Tomasz S
Format: Artikel
Sprache:eng
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Zusammenfassung:A high performance, snapshot Image Mapping Spectrometer was developed that provides fast image acquisition (100 Hz) of 16 bit hyperspectral data cubes (210x210x46) over a spectral range of 515-842 nm. Essential details of the opto-mechanical design are presented. Spectral accuracy, precision, and image reconstruction metrics such as resolution are discussed. Fluorescently stained cell samples were used to directly compare the data obtained using newly developed and the reference image mapping spectrometer. Additional experimental results are provided to demonstrate the abilities of the new spectrometer to acquire highly-resolved, motion-artifact-free hyperspectral images at high temporal sampling rates.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.27.001597