Growth of E. coli in Liquid Medium

We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a h...

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Veröffentlicht in:	Current protocols in molecular biology (Print) 2019-01, Vol.125 (1), p.e81-n/a
Hauptverfasser:	Elbing, Karen L., Brent, Roger
Format:	Artikel
Sprache:	eng
Schlagworte:	aeration cell counting Colony Count, Microbial - instrumentation Colony Count, Microbial - methods Culture Media - chemistry Culture Media - metabolism Escherichia coli - chemistry Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli K‐12 hemacytometer spectrophotometer Spectrophotometry - methods
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Zusammenfassung:	We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a hemacytometer or “count slide.” © 2018 by John Wiley & Sons, Inc.
ISSN:	1934-3639 1934-3647
DOI:	10.1002/cpmb.81