Genetically encoded RNA-based sensors for intracellular imaging of silver ions

Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag+) is critical in these antibacterial effects. The direct cellular measurement of Ag+ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to imag...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2019-01, Vol.55 (5), p.707-710
Hauptverfasser: Yu, Qikun, Shi, Jing, Mudiyanselage, Aruni P K K Karunanayake, Wu, Rigumula, Zhao, Bin, Zhou, Ming, You, Mingxu
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Sprache:eng
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Zusammenfassung:Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag+) is critical in these antibacterial effects. The direct cellular measurement of Ag+ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag+ in live bacterial cells. The sensor is designed by introducing a cytosine-Ag+-cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag+ induces the folding of Broccoli and activates a fluorescence signal. This sensor can be genetically encoded to measure the cellular flux and antibacterial effect of Ag+.
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc08796b