Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells

The aim of the study was to investigate the effects of miRNA-101 and miRNA-345 on HBV replication and liver cancer cell growth. qPCR was performed to detect the expression of miRNA-101 and miRNA-345. The expression of HBV RNA was detected by PCR. The expression of HbsAg was detected using ELISA. BEL...

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Veröffentlicht in:	Oncology letters 2019-01, Vol.17 (1), p.1167-1171
Hauptverfasser:	Ding, Aikun, Wang, Cuiyun, Zhang, Lihua
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Bioengineering Biotechnology Care and treatment Cell culture Cell growth Deoxyribonucleic acid DNA Gene expression Genetic aspects Health aspects Hepatitis Hepatitis B virus Infectious diseases Kinases Liver cancer MicroRNA Oncology Risk factors Studies Virus replication
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Zusammenfassung:	The aim of the study was to investigate the effects of miRNA-101 and miRNA-345 on HBV replication and liver cancer cell growth. qPCR was performed to detect the expression of miRNA-101 and miRNA-345. The expression of HBV RNA was detected by PCR. The expression of HbsAg was detected using ELISA. BEL-7404 cell line proliferation was detected by MTT assay. The expression levels of miR-101 and miR-345 in BEL-7404 pSUPER.neo-miR-101 group and BEL-7404 pSUPER.neo-miR-345 group were significantly higher than those in BEL-7404 pSUPER.neo group (P
ISSN:	1792-1074 1792-1082
DOI:	10.3892/ol.2018.9669