Targeted homing of CCR2-overexpressing mesenchymal stromal cells to ischemic brain enhances post-stroke recovery partially through PRDX4-mediated blood-brain barrier preservation

Mesenchymal stromal cells (MSCs) are emerging as a novel therapeutic strategy for the acute ischemic stroke (AIS). However, the poor targeted migration and low engraftment in ischemic lesions restrict their treatment efficacy. The ischemic brain lesions express a specific chemokine profile, while cu...

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Veröffentlicht in:Theranostics 2018-01, Vol.8 (21), p.5929-5944
Hauptverfasser: Huang, Yinong, Wang, Jiancheng, Cai, Jianye, Qiu, Yuan, Zheng, Haiqing, Lai, Xiaofan, Sui, Xin, Wang, Yi, Lu, Qiying, Zhang, Yanan, Yuan, Meng, Gong, Jin, Cai, Wei, Liu, Xin, Shan, Yilong, Deng, Zhezhi, Shi, Yue, Shu, Yaqing, Zhang, Lei, Qiu, Wei, Peng, Lisheng, Ren, Jie, Lu, Zhengqi, Xiang, Andy Peng
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Sprache:eng
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Zusammenfassung:Mesenchymal stromal cells (MSCs) are emerging as a novel therapeutic strategy for the acute ischemic stroke (AIS). However, the poor targeted migration and low engraftment in ischemic lesions restrict their treatment efficacy. The ischemic brain lesions express a specific chemokine profile, while cultured MSCs lack the set of corresponding receptors. Thus, we hypothesize that overexpression of certain chemokine receptor might help in MSCs homing and improve therapeutic efficacy. Using the middle cerebral artery occlusion (MCAO) model of ischemic stroke, we identified that CCL2 is one of the most highly expressed chemokines in the ipsilateral hemisphere. Then, we genetically transduced the corresponding receptor, CCR2 to the MSCs and quantified the cell retention of MSC compared to the MSC control. MSC exhibited significantly enhanced migration to the ischemic lesions and improved the neurological outcomes. Brain edema and blood-brain barrier (BBB) leakage levels were also found to be much lower in the MSC -treated rats than the MSC group. Moreover, this BBB protection led to reduced inflammation infiltration and reactive oxygen species (ROS) generation. Similar results were also confirmed using the BBB model. Furthermore, genome-wide RNA sequencing (RNA-seq) analysis revealed that peroxiredoxin4 (PRDX4) was highly expressed in MSCs, which mainly contributed to their antioxidant impacts on MCAO rats and oxygen-glucose deprivation (OGD)-treated endothelium. Taken together, this study suggests that overexpression of CCR2 on MSCs enhances their targeted migration to the ischemic hemisphere and improves the therapeutic outcomes, which is attributed to the PRDX4-mediated BBB preservation.
ISSN:1838-7640
1838-7640
DOI:10.7150/thno.28029