Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states o...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2018-12, Vol.54 (97), p.13702-13705
Hauptverfasser: Hellwig, Nils, Peetz, Oliver, Ahdash, Zainab, Tascón, Igor, Booth, Paula J, Mikusevic, Vedrana, Diskowski, Marina, Politis, Argyris, Hellmich, Yvonne, Hänelt, Inga, Reading, Eamonn, Morgner, Nina
Format: Artikel
Sprache:eng
Schlagworte:
Chemistry
Lipid Bilayers - chemistry
Lipids
Lipids - chemistry
Maleates - chemistry
Maleic acid
Mass Spectrometry
Membrane Proteins - analysis
Models, Molecular
Particle Size
Proteins
Styrene - chemistry
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Zusammenfassung:Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc06284f