MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7
The objective of this study was to detect the expression of MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and to investigate the effect of its expression on the progression of NSCLC. Real-time fluorescent quantitative PCR was used to detect the relative expression of MiR-21-5p in 118 NSCL...
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| description | The objective of this study was to detect the expression of MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and to investigate the effect of its expression on the progression of NSCLC.
Real-time fluorescent quantitative PCR was used to detect the relative expression of MiR-21-5p in 118 NSCLC tumor tissues to their adjacent normal tissues. The expressions of SMAD7, MMP-9, E-cadherin, and vimentin proteins were detected by Western blotting or immunohistochemistry. Cell colony formation, scratch, and Transwell assays were used to detect the proliferation, migration, and invasion ability of A549 cells, respectively.
MiR-21-5p was highly expressed in the tumor tissues of NSCLC patients, and its expression was significantly correlated with the clinical classification of NSCLC patients (χ
=7.154,
=0.007), tumor size (χ
=4.372,
=0.037), differentiation (χ
=13.713,
=0.001), lymph node metastasis (χ
=5.101,
=0.024), distant metastasis (χ
=12.599,
=0.000), and TNM stage (χ
=6.344,
=0.012), whereas it was positively correlated with the expression of SMAD7 protein (
=0.669, |
| doi_str_mv | 10.2147/OTT.S172393 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6276624</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A583695267</galeid><sourcerecordid>A583695267</sourcerecordid><originalsourceid>FETCH-LOGICAL-c549t-4fdb86cbfa194e846eb7d1415f2873e8c8bba79cc5c41fdfa0439c10fa214d3</originalsourceid><addsrcrecordid>eNptkt2L1DAUxYso7rr65LsUBBGkY_PdvgjD-gm7LDjzHtL0ppMlTcakFfe_N2XHdUYkD0lufuckOdyieInqFUZUvL_ZblcbJDBpyaPiHCHRVLwl9eOj9VnxLKXbuua8wfRpcUZqxhvKxXlhru33CqOK7ct9DGOYIJXTDpbNECElG3wZTOmDr9KonKs0OFe62Q-lVl5DLLu7MsIwOzXZXFy08Gt_JN1crz-K58UTo1yCF4f5oth8_rS9_Fpd3Xz5drm-qjSj7VRR03cN151RqKWQXwid6BFFzOBGEGh003VKtFozTZHpjaopaTWqjcpJ9OSi-HDvup-7EXoNforKyX20o4p3MigrT0-83ckh_JQcC84xzQZvDwYx_JghTXK0afmx8hDmJDFiLcGMEpLR1_-gt2GOPn9OYkwxJwwR_pcalANpvQn5Xr2YyjVrCG8Z5iJTq_9QefQwWh08GJvrJ4I3R4IdKDftUnDzlDNPp-C7e1DHkFIE8xAGquXSPTJ3jzx0T6ZfHef3wP5pF_Ib19a-Wg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2242635136</pqid></control><display><type>article</type><title>MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7</title><source>Taylor & Francis Open Access</source><source>DOVE Medical Press Journals</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Li, Xiangpan ; Wu, Xiaofei</creator><creatorcontrib>Li, Xiangpan ; Wu, Xiaofei</creatorcontrib><description>The objective of this study was to detect the expression of MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and to investigate the effect of its expression on the progression of NSCLC.
Real-time fluorescent quantitative PCR was used to detect the relative expression of MiR-21-5p in 118 NSCLC tumor tissues to their adjacent normal tissues. The expressions of SMAD7, MMP-9, E-cadherin, and vimentin proteins were detected by Western blotting or immunohistochemistry. Cell colony formation, scratch, and Transwell assays were used to detect the proliferation, migration, and invasion ability of A549 cells, respectively.
MiR-21-5p was highly expressed in the tumor tissues of NSCLC patients, and its expression was significantly correlated with the clinical classification of NSCLC patients (χ
=7.154,
=0.007), tumor size (χ
=4.372,
=0.037), differentiation (χ
=13.713,
=0.001), lymph node metastasis (χ
=5.101,
=0.024), distant metastasis (χ
=12.599,
=0.000), and TNM stage (χ
=6.344,
=0.012), whereas it was positively correlated with the expression of SMAD7 protein (
=0.669,
<0.05). The results of the luciferase gene reporter system showed that MiR-21-5p targeted and promoted the expression of
gene, which enhanced NSCLC cell proliferation. Furthermore, MiR-21-5p promoted the expressions of MMP-9 and vimentin proteins as well as inhibited the expression of E-cadherin protein, which is associated with an elevated SMAD7 protein expression and enhanced the invasion/migration ability of NSCLC cells.
MiR-21-5p was highly expressed in NSCLC tumor tissues, and its high expression could promote NSCLC progression by targeting the expression of SMAD7.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S172393</identifier><identifier>PMID: 30568467</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Anopheles ; Apoptosis ; Binding sites ; Biomarkers ; Biotechnology ; Breast cancer ; Cancer metastasis ; Development and progression ; Gene amplification ; Gene expression ; Genes ; Genomics ; Hospitals ; Immunohistochemistry ; Luciferase ; Lung cancer ; Medical prognosis ; MicroRNAs ; Non-small cell lung cancer ; Non-small cell lung carcinoma ; Polymerase chain reaction ; Proteins ; Rapid Communication ; Tumors</subject><ispartof>OncoTargets and therapy, 2018-01, Vol.11, p.8445-8454</ispartof><rights>COPYRIGHT 2018 Dove Medical Press Limited</rights><rights>2018. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Li and Wu. This work is published and licensed by Dove Medical Press Limited 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-4fdb86cbfa194e846eb7d1415f2873e8c8bba79cc5c41fdfa0439c10fa214d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276624/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276624/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3861,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30568467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiangpan</creatorcontrib><creatorcontrib>Wu, Xiaofei</creatorcontrib><title>MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>The objective of this study was to detect the expression of MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and to investigate the effect of its expression on the progression of NSCLC.
Real-time fluorescent quantitative PCR was used to detect the relative expression of MiR-21-5p in 118 NSCLC tumor tissues to their adjacent normal tissues. The expressions of SMAD7, MMP-9, E-cadherin, and vimentin proteins were detected by Western blotting or immunohistochemistry. Cell colony formation, scratch, and Transwell assays were used to detect the proliferation, migration, and invasion ability of A549 cells, respectively.
MiR-21-5p was highly expressed in the tumor tissues of NSCLC patients, and its expression was significantly correlated with the clinical classification of NSCLC patients (χ
=7.154,
=0.007), tumor size (χ
=4.372,
=0.037), differentiation (χ
=13.713,
=0.001), lymph node metastasis (χ
=5.101,
=0.024), distant metastasis (χ
=12.599,
=0.000), and TNM stage (χ
=6.344,
=0.012), whereas it was positively correlated with the expression of SMAD7 protein (
=0.669,
<0.05). The results of the luciferase gene reporter system showed that MiR-21-5p targeted and promoted the expression of
gene, which enhanced NSCLC cell proliferation. Furthermore, MiR-21-5p promoted the expressions of MMP-9 and vimentin proteins as well as inhibited the expression of E-cadherin protein, which is associated with an elevated SMAD7 protein expression and enhanced the invasion/migration ability of NSCLC cells.
MiR-21-5p was highly expressed in NSCLC tumor tissues, and its high expression could promote NSCLC progression by targeting the expression of SMAD7.</description><subject>Anopheles</subject><subject>Apoptosis</subject><subject>Binding sites</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cancer metastasis</subject><subject>Development and progression</subject><subject>Gene amplification</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomics</subject><subject>Hospitals</subject><subject>Immunohistochemistry</subject><subject>Luciferase</subject><subject>Lung cancer</subject><subject>Medical prognosis</subject><subject>MicroRNAs</subject><subject>Non-small cell lung cancer</subject><subject>Non-small cell lung carcinoma</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Rapid Communication</subject><subject>Tumors</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkt2L1DAUxYso7rr65LsUBBGkY_PdvgjD-gm7LDjzHtL0ppMlTcakFfe_N2XHdUYkD0lufuckOdyieInqFUZUvL_ZblcbJDBpyaPiHCHRVLwl9eOj9VnxLKXbuua8wfRpcUZqxhvKxXlhru33CqOK7ct9DGOYIJXTDpbNECElG3wZTOmDr9KonKs0OFe62Q-lVl5DLLu7MsIwOzXZXFy08Gt_JN1crz-K58UTo1yCF4f5oth8_rS9_Fpd3Xz5drm-qjSj7VRR03cN151RqKWQXwid6BFFzOBGEGh003VKtFozTZHpjaopaTWqjcpJ9OSi-HDvup-7EXoNforKyX20o4p3MigrT0-83ckh_JQcC84xzQZvDwYx_JghTXK0afmx8hDmJDFiLcGMEpLR1_-gt2GOPn9OYkwxJwwR_pcalANpvQn5Xr2YyjVrCG8Z5iJTq_9QefQwWh08GJvrJ4I3R4IdKDftUnDzlDNPp-C7e1DHkFIE8xAGquXSPTJ3jzx0T6ZfHef3wP5pF_Ib19a-Wg</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Li, Xiangpan</creator><creator>Wu, Xiaofei</creator><general>Dove Medical Press Limited</general><general>Taylor & Francis Ltd</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180101</creationdate><title>MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7</title><author>Li, Xiangpan ; Wu, Xiaofei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-4fdb86cbfa194e846eb7d1415f2873e8c8bba79cc5c41fdfa0439c10fa214d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anopheles</topic><topic>Apoptosis</topic><topic>Binding sites</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cancer metastasis</topic><topic>Development and progression</topic><topic>Gene amplification</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomics</topic><topic>Hospitals</topic><topic>Immunohistochemistry</topic><topic>Luciferase</topic><topic>Lung cancer</topic><topic>Medical prognosis</topic><topic>MicroRNAs</topic><topic>Non-small cell lung cancer</topic><topic>Non-small cell lung carcinoma</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Rapid Communication</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiangpan</creatorcontrib><creatorcontrib>Wu, Xiaofei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiangpan</au><au>Wu, Xiaofei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>11</volume><spage>8445</spage><epage>8454</epage><pages>8445-8454</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>The objective of this study was to detect the expression of MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and to investigate the effect of its expression on the progression of NSCLC.
Real-time fluorescent quantitative PCR was used to detect the relative expression of MiR-21-5p in 118 NSCLC tumor tissues to their adjacent normal tissues. The expressions of SMAD7, MMP-9, E-cadherin, and vimentin proteins were detected by Western blotting or immunohistochemistry. Cell colony formation, scratch, and Transwell assays were used to detect the proliferation, migration, and invasion ability of A549 cells, respectively.
MiR-21-5p was highly expressed in the tumor tissues of NSCLC patients, and its expression was significantly correlated with the clinical classification of NSCLC patients (χ
=7.154,
=0.007), tumor size (χ
=4.372,
=0.037), differentiation (χ
=13.713,
=0.001), lymph node metastasis (χ
=5.101,
=0.024), distant metastasis (χ
=12.599,
=0.000), and TNM stage (χ
=6.344,
=0.012), whereas it was positively correlated with the expression of SMAD7 protein (
=0.669,
<0.05). The results of the luciferase gene reporter system showed that MiR-21-5p targeted and promoted the expression of
gene, which enhanced NSCLC cell proliferation. Furthermore, MiR-21-5p promoted the expressions of MMP-9 and vimentin proteins as well as inhibited the expression of E-cadherin protein, which is associated with an elevated SMAD7 protein expression and enhanced the invasion/migration ability of NSCLC cells.
MiR-21-5p was highly expressed in NSCLC tumor tissues, and its high expression could promote NSCLC progression by targeting the expression of SMAD7.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>30568467</pmid><doi>10.2147/OTT.S172393</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | OncoTargets and therapy, 2018-01, Vol.11, p.8445-8454 |
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| language | eng |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Anopheles Apoptosis Binding sites Biomarkers Biotechnology Breast cancer Cancer metastasis Development and progression Gene amplification Gene expression Genes Genomics Hospitals Immunohistochemistry Luciferase Lung cancer Medical prognosis MicroRNAs Non-small cell lung cancer Non-small cell lung carcinoma Polymerase chain reaction Proteins Rapid Communication Tumors |
| title | MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7 |
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