MiR-21-5p promotes the progression of non-small-cell lung cancer by regulating the expression of SMAD7

The objective of this study was to detect the expression of MiR-21-5p in non-small-cell lung cancer (NSCLC) tissues, and to investigate the effect of its expression on the progression of NSCLC. Real-time fluorescent quantitative PCR was used to detect the relative expression of MiR-21-5p in 118 NSCLC tumor tissues to their adjacent normal tissues. The expressions of SMAD7, MMP-9, E-cadherin, and vimentin proteins were detected by Western blotting or immunohistochemistry. Cell colony formation, scratch, and Transwell assays were used to detect the proliferation, migration, and invasion ability of A549 cells, respectively. MiR-21-5p was highly expressed in the tumor tissues of NSCLC patients, and its expression was significantly correlated with the clinical classification of NSCLC patients (χ =7.154, =0.007), tumor size (χ =4.372, =0.037), differentiation (χ =13.713, =0.001), lymph node metastasis (χ =5.101, =0.024), distant metastasis (χ =12.599, =0.000), and TNM stage (χ =6.344, =0.012), whereas it was positively correlated with the expression of SMAD7 protein ( =0.669,