Sucrose Signaling Regulates Anthocyanin Biosynthesis Through a MAPK Cascade in Arabidopsis thaliana

Anthocyanin accumulation specifically depends on sucrose (Suc) signaling. However, the molecular basis of this process remains unknown. In this study, pull-down assays identified ETHYLENE-INSENSITIVE3 (EIN3), a component of both sugar signaling or/and metabolism. This protein interacted with YDA, and the physiological relevance of this interaction was confirmed by co-immunoprecipitation, yeast two-hybrid (Y2H) assay, and bimolecular fluorescence complementation. ( ) double-mutant seedlings, but not seedlings, showed anthocyanin accumulation. Furthermore, suppressed anthocyanin accumulation in plants. Thus, may form a sugar-mediated gene cascade integral to the regulation of anthocyanin accumulation. Moreover, the gene cascade module directly targeted the promoter of ( ) by direct EIN3 binding. Collectively, our data inferred a molecular model where the signaling cascade of the YDA-EIN3-TT8 appeared to target via EIN3, thereby modulating Suc signaling-mediated anthocyanin accumulation.