The Role of CD38 on the Function of Regulatory B Cells in a Murine Model of Lupus

Previous work from our group has shown that mice develop a milder pristane-induced lupus disease than WT or counterparts, demonstrating a new role for CD38 in promoting aberrant inflammation and lupus-like autoimmunity via a Transient Receptor Potential Melastatin 2 (TRPM2)-dependent apoptosis-driven mechanism. In this study we asked whether CD38 may play a role in the expression and function of regulatory B cells (IL-10-producing B cells or B10 cells). In pristane-treated mice the frequency of spleen CD19⁺CD1d CD5⁺ B cells, which are highly enriched in B10 cells, was significantly increased in splenocytes compared to WT, while the frequency of peritoneal plasmacytoid dendritic cells (pDCs), which are major type I Interferon (IFN) producers, was greatly diminished. The low proportion of pDCs correlated with lower amounts of IFN-α in the peritoneal lavage fluids of the mice than of WT and mice. Functional ex vivo assays showed increased frequencies of IL-10-producing B cells in splenocytes than in WT upon stimulation with an agonist anti-CD40 mAb. Overall these results strongly suggest that mice are better suited than WT mice to generate and expand regulatory B10 cells following the appropriate stimulation.