GENE THERAPY EFFECTS ON STRUCTURAL CHANGES IN VARIOUS ORGANS OF YOUNG AND OLD MICE WITH DIABETES

The aim was to study structural, ultrastructural and histochemical changes (light, electron microscopy and morphometry) of the murine pancreatic gland (PG), heart, kidney, liver, spleen and brain and apoptosis in these organs (TUNEL-method) at experimental diabetes mellitus (DM) and its correction w...

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Veröffentlicht in:Innovation in aging 2017-07, Vol.1 (suppl_1), p.897-897
Hauptverfasser: Bezrukov, V., Kvitnitskaya-Ryzhova, T., Mikhalskiy, S., Lugovskoy, S., Klymenko, P., Toporova, O.
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Sprache:eng
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Zusammenfassung:The aim was to study structural, ultrastructural and histochemical changes (light, electron microscopy and morphometry) of the murine pancreatic gland (PG), heart, kidney, liver, spleen and brain and apoptosis in these organs (TUNEL-method) at experimental diabetes mellitus (DM) and its correction with gene therapy (GT) in young (3–5 mos) and old (20 mos) C57BL/6j mice. DM was modeled by injecting Streptozotocin (40 mg/kg) during 5 days. Four weeks after the development of stable hyperglycemia, the plasmid vector of human preproinsulin (PEI-pDNA complex) was injected into animal livers. The animals were sacrificed in 30 days after plasmid injection. After the GT the young animals displayed some normalization of PG and internal organs structure (development of compensatory-adaptive processes in them). We observed the increase of the pancreatic islets quantity, their specific volume in the PG tissue and higher functional activity of beta-cells along with the decrease of the apoptosis level, being combined with a definite normalization of blood glucose. The old animals displayed a significant worsening of study parameters, leading to the destructive and dystrophic changes and development of pathological processes (insulitis) as well as to greater intensity of apoptosis. The effectiveness of GT at experimental DM appeared to be age-dependant. Hence age factor should be taken into consideration in developing DM therapy strategy.
ISSN:2399-5300
2399-5300
DOI:10.1093/geroni/igx004.3217