Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells

Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cance...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular medicine reports 2018-11, Vol.18 (5), p.4381-4387
Hauptverfasser: Wang, Xi, Chen, Hui, Wen, Yiping, Yang, Xiaoxin, Han, Qing, Jiang, Ping, Huang, Zaiju, Cai, Jing, Wang, Zehua
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 4387
container_issue 5
container_start_page 4381
container_title Molecular medicine reports
container_volume 18
creator Wang, Xi
Chen, Hui
Wen, Yiping
Yang, Xiaoxin
Han, Qing
Jiang, Ping
Huang, Zaiju
Cai, Jing
Wang, Zehua
description Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.
doi_str_mv 10.3892/mmr.2018.9452
format Article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6172369</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A559686453</galeid><sourcerecordid>A559686453</sourcerecordid><originalsourceid>FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</originalsourceid><addsrcrecordid>eNptks9KHTEUxkNpqXrbpVsZ6Kabuc3fSbIRxKotCG7adcjNnGhkJhmTuUJ3fYW-Yp_EDF5tLSWEHJLf-U5O8iF0SPCaKU0_jWNeU0zUWnNBX6F9IjVpGcb89S6mWss9dFDKLcadoEK_RXsMU0ok4_vo6nNwkBvrPbi5NC6UabBziL9__hqhD3aGvrFTmuZUQmlCbGAK8w0MwQ5Nurc52Ng4GxcNB8NQ3qE33g4F3u_WFfp-fvbt9Et7eXXx9fTksnVc0bnluFbkEjyT3HtttdhogjfMY42Zshqc8J3ssVYSeup710kuNLZac9gop9gKHT_qTttNvaiDOGc7mCmH0eYfJtlgXp7EcGOu073piKSs01Xg404gp7stlNmMoSwt2AhpWwwlWFHBdJ0r9OEf9DZtc6ztVYp0UhHO1R_q2g5gQvSp1nWLqDkRQneq44JVav0fqo4exuBSBB_q_ouE9jHB5VRKBv_cI8FmcYCpDjCLA8zigMof_f0wz_TTl7MHgH2tIw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2116781448</pqid></control><display><type>article</type><title>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Wang, Xi ; Chen, Hui ; Wen, Yiping ; Yang, Xiaoxin ; Han, Qing ; Jiang, Ping ; Huang, Zaiju ; Cai, Jing ; Wang, Zehua</creator><creatorcontrib>Wang, Xi ; Chen, Hui ; Wen, Yiping ; Yang, Xiaoxin ; Han, Qing ; Jiang, Ping ; Huang, Zaiju ; Cai, Jing ; Wang, Zehua</creatorcontrib><description>Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2018.9452</identifier><identifier>PMID: 30221734</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Apoptosis ; Apoptosis - drug effects ; Carcinoma, Ovarian Epithelial - drug therapy ; Carcinoma, Ovarian Epithelial - genetics ; Carcinoma, Ovarian Epithelial - pathology ; Care and treatment ; Caspase ; Caspase-3 ; Caspase-9 ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Chemoresistance ; Chemotherapy ; Cisplatin ; Cisplatin - pharmacology ; DEAD-box RNA Helicases - genetics ; Development and progression ; Drug resistance ; Drug Resistance, Neoplasm - genetics ; Epithelial tumors ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Knockdown Techniques ; Genetic aspects ; Health aspects ; Humans ; Laboratories ; MicroRNA ; MicroRNAs ; Mutation ; Neoplasm Proteins - genetics ; Ovarian cancer ; p53 Protein ; Proteins ; Ribonuclease III - genetics ; Ribonucleic acid ; RNA ; Tumors</subject><ispartof>Molecular medicine reports, 2018-11, Vol.18 (5), p.4381-4387</ispartof><rights>COPYRIGHT 2018 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright: © Wang et al. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</citedby><cites>FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30221734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Wen, Yiping</creatorcontrib><creatorcontrib>Yang, Xiaoxin</creatorcontrib><creatorcontrib>Han, Qing</creatorcontrib><creatorcontrib>Jiang, Ping</creatorcontrib><creatorcontrib>Huang, Zaiju</creatorcontrib><creatorcontrib>Cai, Jing</creatorcontrib><creatorcontrib>Wang, Zehua</creatorcontrib><title>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.</description><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Carcinoma, Ovarian Epithelial - drug therapy</subject><subject>Carcinoma, Ovarian Epithelial - genetics</subject><subject>Carcinoma, Ovarian Epithelial - pathology</subject><subject>Care and treatment</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Caspase-9</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Chemoresistance</subject><subject>Chemotherapy</subject><subject>Cisplatin</subject><subject>Cisplatin - pharmacology</subject><subject>DEAD-box RNA Helicases - genetics</subject><subject>Development and progression</subject><subject>Drug resistance</subject><subject>Drug Resistance, Neoplasm - genetics</subject><subject>Epithelial tumors</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Knockdown Techniques</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Laboratories</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Mutation</subject><subject>Neoplasm Proteins - genetics</subject><subject>Ovarian cancer</subject><subject>p53 Protein</subject><subject>Proteins</subject><subject>Ribonuclease III - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Tumors</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptks9KHTEUxkNpqXrbpVsZ6Kabuc3fSbIRxKotCG7adcjNnGhkJhmTuUJ3fYW-Yp_EDF5tLSWEHJLf-U5O8iF0SPCaKU0_jWNeU0zUWnNBX6F9IjVpGcb89S6mWss9dFDKLcadoEK_RXsMU0ok4_vo6nNwkBvrPbi5NC6UabBziL9__hqhD3aGvrFTmuZUQmlCbGAK8w0MwQ5Nurc52Ng4GxcNB8NQ3qE33g4F3u_WFfp-fvbt9Et7eXXx9fTksnVc0bnluFbkEjyT3HtttdhogjfMY42Zshqc8J3ssVYSeup710kuNLZac9gop9gKHT_qTttNvaiDOGc7mCmH0eYfJtlgXp7EcGOu073piKSs01Xg404gp7stlNmMoSwt2AhpWwwlWFHBdJ0r9OEf9DZtc6ztVYp0UhHO1R_q2g5gQvSp1nWLqDkRQneq44JVav0fqo4exuBSBB_q_ouE9jHB5VRKBv_cI8FmcYCpDjCLA8zigMof_f0wz_TTl7MHgH2tIw</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Wang, Xi</creator><creator>Chen, Hui</creator><creator>Wen, Yiping</creator><creator>Yang, Xiaoxin</creator><creator>Han, Qing</creator><creator>Jiang, Ping</creator><creator>Huang, Zaiju</creator><creator>Cai, Jing</creator><creator>Wang, Zehua</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181101</creationdate><title>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</title><author>Wang, Xi ; Chen, Hui ; Wen, Yiping ; Yang, Xiaoxin ; Han, Qing ; Jiang, Ping ; Huang, Zaiju ; Cai, Jing ; Wang, Zehua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Carcinoma, Ovarian Epithelial - drug therapy</topic><topic>Carcinoma, Ovarian Epithelial - genetics</topic><topic>Carcinoma, Ovarian Epithelial - pathology</topic><topic>Care and treatment</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Caspase-9</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Chemoresistance</topic><topic>Chemotherapy</topic><topic>Cisplatin</topic><topic>Cisplatin - pharmacology</topic><topic>DEAD-box RNA Helicases - genetics</topic><topic>Development and progression</topic><topic>Drug resistance</topic><topic>Drug Resistance, Neoplasm - genetics</topic><topic>Epithelial tumors</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Gene Knockdown Techniques</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Laboratories</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>Mutation</topic><topic>Neoplasm Proteins - genetics</topic><topic>Ovarian cancer</topic><topic>p53 Protein</topic><topic>Proteins</topic><topic>Ribonuclease III - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Wen, Yiping</creatorcontrib><creatorcontrib>Yang, Xiaoxin</creatorcontrib><creatorcontrib>Han, Qing</creatorcontrib><creatorcontrib>Jiang, Ping</creatorcontrib><creatorcontrib>Huang, Zaiju</creatorcontrib><creatorcontrib>Cai, Jing</creatorcontrib><creatorcontrib>Wang, Zehua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xi</au><au>Chen, Hui</au><au>Wen, Yiping</au><au>Yang, Xiaoxin</au><au>Han, Qing</au><au>Jiang, Ping</au><au>Huang, Zaiju</au><au>Cai, Jing</au><au>Wang, Zehua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>18</volume><issue>5</issue><spage>4381</spage><epage>4387</epage><pages>4381-4387</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>30221734</pmid><doi>10.3892/mmr.2018.9452</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1791-2997
ispartof Molecular medicine reports, 2018-11, Vol.18 (5), p.4381-4387
issn 1791-2997
1791-3004
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6172369
source Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Apoptosis
Apoptosis - drug effects
Carcinoma, Ovarian Epithelial - drug therapy
Carcinoma, Ovarian Epithelial - genetics
Carcinoma, Ovarian Epithelial - pathology
Care and treatment
Caspase
Caspase-3
Caspase-9
Cell Line, Tumor
Cell Proliferation - drug effects
Chemoresistance
Chemotherapy
Cisplatin
Cisplatin - pharmacology
DEAD-box RNA Helicases - genetics
Development and progression
Drug resistance
Drug Resistance, Neoplasm - genetics
Epithelial tumors
Female
Gene expression
Gene Expression Regulation, Neoplastic - drug effects
Gene Knockdown Techniques
Genetic aspects
Health aspects
Humans
Laboratories
MicroRNA
MicroRNAs
Mutation
Neoplasm Proteins - genetics
Ovarian cancer
p53 Protein
Proteins
Ribonuclease III - genetics
Ribonucleic acid
RNA
Tumors
title Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T19%3A22%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dicer%20affects%20cisplatin%E2%80%91mediated%20apoptosis%20in%20epithelial%20ovarian%20cancer%20cells&rft.jtitle=Molecular%20medicine%20reports&rft.au=Wang,%20Xi&rft.date=2018-11-01&rft.volume=18&rft.issue=5&rft.spage=4381&rft.epage=4387&rft.pages=4381-4387&rft.issn=1791-2997&rft.eissn=1791-3004&rft_id=info:doi/10.3892/mmr.2018.9452&rft_dat=%3Cgale_pubme%3EA559686453%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2116781448&rft_id=info:pmid/30221734&rft_galeid=A559686453&rfr_iscdi=true