Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells
Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cance...
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Veröffentlicht in: | Molecular medicine reports 2018-11, Vol.18 (5), p.4381-4387 |
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description | Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer. |
doi_str_mv | 10.3892/mmr.2018.9452 |
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However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2018.9452</identifier><identifier>PMID: 30221734</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Apoptosis ; Apoptosis - drug effects ; Carcinoma, Ovarian Epithelial - drug therapy ; Carcinoma, Ovarian Epithelial - genetics ; Carcinoma, Ovarian Epithelial - pathology ; Care and treatment ; Caspase ; Caspase-3 ; Caspase-9 ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Chemoresistance ; Chemotherapy ; Cisplatin ; Cisplatin - pharmacology ; DEAD-box RNA Helicases - genetics ; Development and progression ; Drug resistance ; Drug Resistance, Neoplasm - genetics ; Epithelial tumors ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Knockdown Techniques ; Genetic aspects ; Health aspects ; Humans ; Laboratories ; MicroRNA ; MicroRNAs ; Mutation ; Neoplasm Proteins - genetics ; Ovarian cancer ; p53 Protein ; Proteins ; Ribonuclease III - genetics ; Ribonucleic acid ; RNA ; Tumors</subject><ispartof>Molecular medicine reports, 2018-11, Vol.18 (5), p.4381-4387</ispartof><rights>COPYRIGHT 2018 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright: © Wang et al. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</citedby><cites>FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30221734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Wen, Yiping</creatorcontrib><creatorcontrib>Yang, Xiaoxin</creatorcontrib><creatorcontrib>Han, Qing</creatorcontrib><creatorcontrib>Jiang, Ping</creatorcontrib><creatorcontrib>Huang, Zaiju</creatorcontrib><creatorcontrib>Cai, Jing</creatorcontrib><creatorcontrib>Wang, Zehua</creatorcontrib><title>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.</description><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Carcinoma, Ovarian Epithelial - drug therapy</subject><subject>Carcinoma, Ovarian Epithelial - genetics</subject><subject>Carcinoma, Ovarian Epithelial - pathology</subject><subject>Care and treatment</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Caspase-9</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Chemoresistance</subject><subject>Chemotherapy</subject><subject>Cisplatin</subject><subject>Cisplatin - pharmacology</subject><subject>DEAD-box RNA Helicases - genetics</subject><subject>Development and progression</subject><subject>Drug resistance</subject><subject>Drug Resistance, Neoplasm - genetics</subject><subject>Epithelial tumors</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Knockdown Techniques</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Laboratories</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Mutation</subject><subject>Neoplasm Proteins - genetics</subject><subject>Ovarian cancer</subject><subject>p53 Protein</subject><subject>Proteins</subject><subject>Ribonuclease III - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Tumors</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptks9KHTEUxkNpqXrbpVsZ6Kabuc3fSbIRxKotCG7adcjNnGhkJhmTuUJ3fYW-Yp_EDF5tLSWEHJLf-U5O8iF0SPCaKU0_jWNeU0zUWnNBX6F9IjVpGcb89S6mWss9dFDKLcadoEK_RXsMU0ok4_vo6nNwkBvrPbi5NC6UabBziL9__hqhD3aGvrFTmuZUQmlCbGAK8w0MwQ5Nurc52Ng4GxcNB8NQ3qE33g4F3u_WFfp-fvbt9Et7eXXx9fTksnVc0bnluFbkEjyT3HtttdhogjfMY42Zshqc8J3ssVYSeup710kuNLZac9gop9gKHT_qTttNvaiDOGc7mCmH0eYfJtlgXp7EcGOu073piKSs01Xg404gp7stlNmMoSwt2AhpWwwlWFHBdJ0r9OEf9DZtc6ztVYp0UhHO1R_q2g5gQvSp1nWLqDkRQneq44JVav0fqo4exuBSBB_q_ouE9jHB5VRKBv_cI8FmcYCpDjCLA8zigMof_f0wz_TTl7MHgH2tIw</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Wang, Xi</creator><creator>Chen, Hui</creator><creator>Wen, Yiping</creator><creator>Yang, Xiaoxin</creator><creator>Han, Qing</creator><creator>Jiang, Ping</creator><creator>Huang, Zaiju</creator><creator>Cai, Jing</creator><creator>Wang, Zehua</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181101</creationdate><title>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</title><author>Wang, Xi ; Chen, Hui ; Wen, Yiping ; Yang, Xiaoxin ; Han, Qing ; Jiang, Ping ; Huang, Zaiju ; Cai, Jing ; Wang, Zehua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-40aff47ef374ff9a95b910b3f09038a9ec5f67d0987ed2fdc674590a994eb8c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Carcinoma, Ovarian Epithelial - drug therapy</topic><topic>Carcinoma, Ovarian Epithelial - genetics</topic><topic>Carcinoma, Ovarian Epithelial - pathology</topic><topic>Care and treatment</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Caspase-9</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Chemoresistance</topic><topic>Chemotherapy</topic><topic>Cisplatin</topic><topic>Cisplatin - pharmacology</topic><topic>DEAD-box RNA Helicases - genetics</topic><topic>Development and progression</topic><topic>Drug resistance</topic><topic>Drug Resistance, Neoplasm - genetics</topic><topic>Epithelial tumors</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Gene Knockdown Techniques</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Laboratories</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>Mutation</topic><topic>Neoplasm Proteins - genetics</topic><topic>Ovarian cancer</topic><topic>p53 Protein</topic><topic>Proteins</topic><topic>Ribonuclease III - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Wen, Yiping</creatorcontrib><creatorcontrib>Yang, Xiaoxin</creatorcontrib><creatorcontrib>Han, Qing</creatorcontrib><creatorcontrib>Jiang, Ping</creatorcontrib><creatorcontrib>Huang, Zaiju</creatorcontrib><creatorcontrib>Cai, Jing</creatorcontrib><creatorcontrib>Wang, Zehua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xi</au><au>Chen, Hui</au><au>Wen, Yiping</au><au>Yang, Xiaoxin</au><au>Han, Qing</au><au>Jiang, Ping</au><au>Huang, Zaiju</au><au>Cai, Jing</au><au>Wang, Zehua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>18</volume><issue>5</issue><spage>4381</spage><epage>4387</epage><pages>4381-4387</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Dicer is an essential enzyme that processes micro (mi)-RNA precursors into mature miRNAs, and serves a critical role in cancer development and progression by regulating gene expression. However, the role of Dicer in cisplatin‑mediated apoptosis and chemotherapy resistance in epithelial ovarian cancer (EOC) cells is poorly understood. In the present study, Dicer was expressed at low levels in cisplatin‑resistant A2780 cells when compared with parental cells. In addition, knocking down Dicer using short hairpin RNA decreased the sensitivity of A2780 and CAOV3 cells to cisplatin. Furthermore, downregulating Dicer significantly inhibited cisplatin‑induced apoptosis in ovarian cancer cells, and decreased the levels of proteins involved in apoptosis signaling pathways, including P73, P63, P53, caspase‑9 and caspase‑3. These findings indicated that Dicer may be a promising target for overcoming drug resistance in ovarian cancer.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>30221734</pmid><doi>10.3892/mmr.2018.9452</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Apoptosis Apoptosis - drug effects Carcinoma, Ovarian Epithelial - drug therapy Carcinoma, Ovarian Epithelial - genetics Carcinoma, Ovarian Epithelial - pathology Care and treatment Caspase Caspase-3 Caspase-9 Cell Line, Tumor Cell Proliferation - drug effects Chemoresistance Chemotherapy Cisplatin Cisplatin - pharmacology DEAD-box RNA Helicases - genetics Development and progression Drug resistance Drug Resistance, Neoplasm - genetics Epithelial tumors Female Gene expression Gene Expression Regulation, Neoplastic - drug effects Gene Knockdown Techniques Genetic aspects Health aspects Humans Laboratories MicroRNA MicroRNAs Mutation Neoplasm Proteins - genetics Ovarian cancer p53 Protein Proteins Ribonuclease III - genetics Ribonucleic acid RNA Tumors |
title | Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells |
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