Direct Visualization of the Conformational Dynamics of Single Influenza Hemagglutinin Trimers

Influenza hemagglutinin (HA) is the canonical type I viral envelope glycoprotein and provides a template for the membrane-fusion mechanisms of numerous viruses. The current model of HA-mediated membrane fusion describes a static “spring-loaded” fusion domain (HA2) at neutral pH. Acidic pH triggers a singular irreversible conformational rearrangement in HA2 that fuses viral and cellular membranes. Here, using single-molecule Förster resonance energy transfer (smFRET)-imaging, we directly visualized pH-triggered conformational changes of HA trimers on the viral surface. Our analyses reveal reversible exchange between the pre-fusion and two intermediate conformations of HA2. Acidification of pH and receptor binding shifts the dynamic equilibrium of HA2 in favor of forward progression along the membrane-fusion reaction coordinate. Interaction with the target membrane promotes irreversible transition of HA2 to the post-fusion state. The reversibility of HA2 conformation may protect against transition to the post-fusion state prior to arrival at the target membrane. [Display omitted] •Influenza HA spontaneously samples conformations related to membrane fusion•Interaction with the receptor allosterically induces fusion peptide movement•pH, receptor binding, and interaction with a target membrane regulate HA dynamics•HA can return to the pre-fusion conformation after transient exposure to acidic pH Single-molecule FRET is used to provide real-time visualization of influenza hemagglutinin conformational dynamics on the viral surface during triggering by low pH.