Determination of Helicobacter pylori virulence-associated genes in duodenal ulcer and gastric biopsies

or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for , and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine and genes in strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran. Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran, Iran during January and November 2015. After isolation of from the biopsy culture, genomic DNA was extracted and subsequently used to identify and virulence genes using specific primers. The isolation rate of strains was 65.7% (169/257). The frequency of and was 143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively. In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC patients (p