ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined adva...

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Veröffentlicht in:Nanobiomedicine 2015-01, Vol.2 (Godište 2015), p.10-10
Hauptverfasser: Smerkova, Kristyna, Vlcnovska, Marcela, Dostalova, Simona, Milosavljevic, Vedran, Kopel, Pavel, Vaculovic, Tomas, Krizkova, Sona, Vaculovicova, Marketa, Adam, Vojtech, Kizek, Rene
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Sprache:eng
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Zusammenfassung:Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.
ISSN:1849-5435
1849-5435
DOI:10.5772/61699