Enhancement of Immunoregulatory Function of Modified Bone Marrow Mesenchymal Stem Cells by Targeting SOCS1

Objective. The study aim to investigate the role of microRNA-155 (miR-155) on the immunoregulatory function of bone marrow mesenchymal stem cells (MSCs). Methods. MSCs were isolated from 2-week-old Sprague-Dawley rats and identified by flow cytometry using anti-CD29, anti-CD44, anti-CD34, and anti-C...

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Veröffentlicht in:	BioMed research international 2018-01, Vol.2018 (2018), p.1-10
Hauptverfasser:	Shen, Zhenya, Huang, Haoyue, Teng, Xiaomei, Chen, Yueqiu, Yang, Ziying, Hua, Fei, Zhang, Xiaoming, Zhao, Yunfeng
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antigens, CD - metabolism Apoptosis B cells Biomedical research Bone marrow Bone Marrow Cells - metabolism CD29 antigen CD34 antigen CD4 antigen CD44 antigen CD45 antigen Cell Differentiation - physiology Cells, Cultured Coculture Techniques Cytokines Dendritic cells Differentiation Flow cytometry Forkhead Transcription Factors - metabolism Foxp3 protein GATA-3 protein GATA3 Transcription Factor - metabolism Gene expression Helper cells Identification methods Immunologic Factors - metabolism Immunology Immunoregulation Inhibitors Laboratory animals Leukocytes (mononuclear) Lymphocytes Lymphocytes T Male Mesenchymal Stem Cell Transplantation - methods Mesenchymal stem cells Mesenchymal Stem Cells - metabolism Mesenchyme MicroRNA MicroRNAs MicroRNAs - metabolism miRNA Proteins Rats Rats, Sprague-Dawley Rheumatoid arthritis Ribonucleic acid RNA RNA, Messenger - metabolism Rodents Spleen Stem cell transplantation Stem cells Suppressor of Cytokine Signaling 1 Protein - metabolism T cells T-Box Domain Proteins - metabolism T-Lymphocytes, Helper-Inducer T-Lymphocytes, Regulatory - metabolism Target detection Transcription factors Tumor necrosis factor-TNF
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Zusammenfassung:	Objective. The study aim to investigate the role of microRNA-155 (miR-155) on the immunoregulatory function of bone marrow mesenchymal stem cells (MSCs). Methods. MSCs were isolated from 2-week-old Sprague-Dawley rats and identified by flow cytometry using anti-CD29, anti-CD44, anti-CD34, and anti-CD45 antibodies. MSCs were transfected with miR155-mimics, miR155-inhibitor, and control oligos, respectively, and then cocultured with spleen mononuclear cells (SMCs). The mRNA levels of Th1, Th2, Th17, and Treg cell-specific transcription factors (Tbx21, Gata3, Rorc, and Foxp3, resp.) and the miR-155 target gene SOCS1 were detected by quantitative real-time PCR (qPCR) in SMCs. The proportion of CD4+ FOXP3+ Treg cells was detected by flow cytometry. In addition, the effects of MSCs transfected with miR-155 on the migration of rat SMCs were investigated by transwell chamber. Results. CD29 and CD44 were expressed in MSCs, while CD34 and CD45 were negative. The percentage of CD4+ FOXP3+ Treg cells in the SMC population was significantly higher compared with that noted in SMCs control group (p
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2018/3530647