Simplified qPCR method for detecting excessive mtDNA damage induced by exogenous factors

Highlights • Set of primers allows to determine specific sites of damage in mice mtDNA has been developed. • Method of isolation of the mtDNA from mice tissues has been modified. • In vitro hydrogen-peroxide-induced damage of mtDNA correlated with GTGR-sequence-enriched segments. • Single rotenone i...

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Veröffentlicht in:	Toxicology (Amsterdam) 2017-05, Vol.382, p.67-74
Hauptverfasser:	Gureev, Artem P, Shaforostova, Ekaterina A, Starkov, Anatoly A, Popov, Vasily N
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals biomarkers Brain - metabolism DNA Damage DNA, Mitochondrial - genetics DNA-directed DNA polymerase drugs Emergency genotoxicity Hydrogen peroxide Hydrogen Peroxide - toxicity Liver - metabolism Male mice Mice, Inbred C57BL Mitochondria - metabolism Mitochondrial DNA nuclear genome nucleic acid annealing Oxidative damage Polymerase Chain Reaction - methods quantitative polymerase chain reaction Rotenone Rotenone - toxicity RTGR-sequence toxins
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Beschreibung
Zusammenfassung:	Highlights • Set of primers allows to determine specific sites of damage in mice mtDNA has been developed. • Method of isolation of the mtDNA from mice tissues has been modified. • In vitro hydrogen-peroxide-induced damage of mtDNA correlated with GTGR-sequence-enriched segments. • Single rotenone injection caused strong damage to all segments of mtDNA in the midbrain but not in the brain cortex.
ISSN:	0300-483X 1879-3185
DOI:	10.1016/j.tox.2017.03.010