Ectopic RING zinc finger gene from hot pepper induces totally different genes in lettuce and tobacco
Advances in molecular biology have improved crops through transferring genes from one organism to new hosts, and these efforts have raised concerns about potential unexpected outcomes. Here, we provide evidence that a gene with a specific function in one organism can yield completely different effec...
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Veröffentlicht in: | Molecular breeding 2018, Vol.38 (6), p.70-24, Article 70 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Advances in molecular biology have improved crops through transferring genes from one organism to new hosts, and these efforts have raised concerns about potential unexpected outcomes. Here, we provide evidence that a gene with a specific function in one organism can yield completely different effects in a new host.
CaRZFP1
is a C3HC4-type RING zinc finger protein gene previously isolated from a cDNA library for heat-stressed hot pepper. In our previous work investigating in vivo
CaRZFP1
function, we transferred
CaRZFP1
into tobacco; transgenic tobacco exhibited enhanced growth and tolerance to abiotic stresses. As further analysis of
CaRZFP1
ectopic expression in a heterologous host plant, here we mobilized and constitutively overexpressed
CaRZFP1
in lettuce. In contrast to tobacco, transgenic lettuce exhibited poorer growth and delayed flowering compared with vector-only controls. To identify genes that might be involved in this phenotypic effect, transcriptome analyses on transgenic plants of both species were performed, uncovering dozens of genes that reflect the different outcomes between tobacco and lettuce. These included protein kinase, transcriptional factor, transporter protein, hormone and metabolism-related genes, and some unannotated genes. The opposite effects of
CaRZFP1
ectopic expression in lettuce and tobacco address concerns of unexpectedly different outcomes in different host species. |
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ISSN: | 1380-3743 1572-9788 |
DOI: | 10.1007/s11032-018-0812-3 |