Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model
The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopu...
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| Veröffentlicht in: | Cell death & disease 2018-05, Vol.9 (5), p.502-19, Article 502 |
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| creator | Fan, Jia-Rong Lee, Hsu-Tung Lee, Wei Lin, Chen-Huan Hsu, Chun Y. Hsieh, Chia-Hung Shyu, Woei-Cherng |
| description | The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of
a
dult
p
luripotent-like
o
lfactory
s
tem
c
ells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7
+/+
mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7
−/−
mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7
−/−
APOSCs underwent premature senescence, whereas CBX7
+/+
APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment. |
| doi_str_mv | 10.1038/s41419-018-0519-8 |
| format | Article |
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a
dult
p
luripotent-like
o
lfactory
s
tem
c
ells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7
+/+
mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7
−/−
mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7
−/−
APOSCs underwent premature senescence, whereas CBX7
+/+
APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment.</description><identifier>ISSN: 2041-4889</identifier><identifier>EISSN: 2041-4889</identifier><identifier>DOI: 10.1038/s41419-018-0519-8</identifier><identifier>PMID: 29717132</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/100 ; 13/95 ; Alkaline phosphatase ; Antibodies ; Autografts ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Cell Culture ; Cell self-renewal ; Immunology ; Life Sciences ; Mucosa ; Neurogenesis ; Neurological complications ; Oct-4 protein ; Olfactory epithelium ; Pluripotency ; Regeneration ; Senescence ; Stem cell transplantation ; Stem cells ; Stroke</subject><ispartof>Cell death & disease, 2018-05, Vol.9 (5), p.502-19, Article 502</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-911e4c493c55621cdc0a43f8ff1be72e74dc6c92f7c631715b81d73a1829e0c43</citedby><cites>FETCH-LOGICAL-c400t-911e4c493c55621cdc0a43f8ff1be72e74dc6c92f7c631715b81d73a1829e0c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931587/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931587/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29717132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Jia-Rong</creatorcontrib><creatorcontrib>Lee, Hsu-Tung</creatorcontrib><creatorcontrib>Lee, Wei</creatorcontrib><creatorcontrib>Lin, Chen-Huan</creatorcontrib><creatorcontrib>Hsu, Chun Y.</creatorcontrib><creatorcontrib>Hsieh, Chia-Hung</creatorcontrib><creatorcontrib>Shyu, Woei-Cherng</creatorcontrib><title>Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model</title><title>Cell death & disease</title><addtitle>Cell Death Dis</addtitle><addtitle>Cell Death Dis</addtitle><description>The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of
a
dult
p
luripotent-like
o
lfactory
s
tem
c
ells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7
+/+
mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7
−/−
mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7
−/−
APOSCs underwent premature senescence, whereas CBX7
+/+
APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment.</description><subject>13/100</subject><subject>13/95</subject><subject>Alkaline phosphatase</subject><subject>Antibodies</subject><subject>Autografts</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell self-renewal</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Mucosa</subject><subject>Neurogenesis</subject><subject>Neurological complications</subject><subject>Oct-4 protein</subject><subject>Olfactory epithelium</subject><subject>Pluripotency</subject><subject>Regeneration</subject><subject>Senescence</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stroke</subject><issn>2041-4889</issn><issn>2041-4889</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UV1LHDEUDaWlivoD-lICvvRlam4-NpkXoS79AsE-KPQtZDOZNTYz2SYZYf-9GdbaVTAvuXDOPTknB6EPQD4DYeosc-DQNgRUQ0Qd1Bt0SAmHhivVvt2bD9BJznekHsYIFYv36IC2EiQweojuf8XixuJNwCkGh2OPlxe_JfYjTm49BVP8uMabMCW_mZl2O1NMN4WCb6fBjHtYaYL_UyVCb2yJaYtzcQO2LoQ86-WSYoWH2LlwjN71JmR38ngfoZtvX6-XP5rLq-8_l18uG8sJKU0L4LjlLbNCLCjYzhLDWa_6HlZOUid5Zxe2pb20C1YTiZWCTjIDiraOWM6O0PlOdzOtBtfZajKZoDfJDyZtdTReP0dGf6vX8V6LloFQsgp8ehRI8e_kctGDz3MkM7o4ZU3rpzJFqrlKPX1BvYtTGmu8yhIcmOBydgQ7lk0x5-T6JzNA9Fys3hWra7F6LlaruvNxP8XTxr8aK4HuCLlC49ql_0-_rvoAQkKvxA</recordid><startdate>20180502</startdate><enddate>20180502</enddate><creator>Fan, Jia-Rong</creator><creator>Lee, Hsu-Tung</creator><creator>Lee, Wei</creator><creator>Lin, Chen-Huan</creator><creator>Hsu, Chun Y.</creator><creator>Hsieh, Chia-Hung</creator><creator>Shyu, Woei-Cherng</creator><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180502</creationdate><title>Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model</title><author>Fan, Jia-Rong ; Lee, Hsu-Tung ; Lee, Wei ; Lin, Chen-Huan ; Hsu, Chun Y. ; Hsieh, Chia-Hung ; Shyu, Woei-Cherng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-911e4c493c55621cdc0a43f8ff1be72e74dc6c92f7c631715b81d73a1829e0c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>13/100</topic><topic>13/95</topic><topic>Alkaline phosphatase</topic><topic>Antibodies</topic><topic>Autografts</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell self-renewal</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Mucosa</topic><topic>Neurogenesis</topic><topic>Neurological complications</topic><topic>Oct-4 protein</topic><topic>Olfactory epithelium</topic><topic>Pluripotency</topic><topic>Regeneration</topic><topic>Senescence</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Stroke</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Jia-Rong</creatorcontrib><creatorcontrib>Lee, Hsu-Tung</creatorcontrib><creatorcontrib>Lee, Wei</creatorcontrib><creatorcontrib>Lin, Chen-Huan</creatorcontrib><creatorcontrib>Hsu, Chun Y.</creatorcontrib><creatorcontrib>Hsieh, Chia-Hung</creatorcontrib><creatorcontrib>Shyu, Woei-Cherng</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell death & disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Jia-Rong</au><au>Lee, Hsu-Tung</au><au>Lee, Wei</au><au>Lin, Chen-Huan</au><au>Hsu, Chun Y.</au><au>Hsieh, Chia-Hung</au><au>Shyu, Woei-Cherng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model</atitle><jtitle>Cell death & disease</jtitle><stitle>Cell Death Dis</stitle><addtitle>Cell Death Dis</addtitle><date>2018-05-02</date><risdate>2018</risdate><volume>9</volume><issue>5</issue><spage>502</spage><epage>19</epage><pages>502-19</pages><artnum>502</artnum><issn>2041-4889</issn><eissn>2041-4889</eissn><abstract>The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of
a
dult
p
luripotent-like
o
lfactory
s
tem
c
ells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7
+/+
mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7
−/−
mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7
−/−
APOSCs underwent premature senescence, whereas CBX7
+/+
APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29717132</pmid><doi>10.1038/s41419-018-0519-8</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | 13/100 13/95 Alkaline phosphatase Antibodies Autografts Biochemistry Biomedical and Life Sciences Cell Biology Cell Culture Cell self-renewal Immunology Life Sciences Mucosa Neurogenesis Neurological complications Oct-4 protein Olfactory epithelium Pluripotency Regeneration Senescence Stem cell transplantation Stem cells Stroke |
| title | Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model |
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