Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model

The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopu...

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Veröffentlicht in:Cell death & disease 2018-05, Vol.9 (5), p.502-19, Article 502
Hauptverfasser: Fan, Jia-Rong, Lee, Hsu-Tung, Lee, Wei, Lin, Chen-Huan, Hsu, Chun Y., Hsieh, Chia-Hung, Shyu, Woei-Cherng
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Sprache:eng
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Zusammenfassung:The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of a dult p luripotent-like o lfactory s tem c ells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7 +/+ mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7 −/− mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7 −/− APOSCs underwent premature senescence, whereas CBX7 +/+ APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment.
ISSN:2041-4889
2041-4889
DOI:10.1038/s41419-018-0519-8