Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi

The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of . With incubation at 37°C, the gene of was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solu...

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Veröffentlicht in:	The American journal of tropical medicine and hygiene 2018-01, Vol.98 (3), p.700-703
Hauptverfasser:	Lai, Meng-Yee, Ooi, Choo-Huck, Lau, Yee-Ling
Format:	Artikel
Sprache:	eng
Schlagworte:	Diagnostic tests Malaria Medical diagnosis Nucleic Acid Amplification Techniques - methods Parasites Plasmodium knowlesi - genetics Plasmodium knowlesi - isolation & purification Reagent Strips Recombinases - genetics Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of . With incubation at 37°C, the gene of was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of . Nonetheless, it was demonstrated that all ( = 41) and other sp. ( = 25) were positive while negative samples ( = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings.
ISSN:	0002-9637 1476-1645
DOI:	10.4269/ajtmh.17-0738