Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps
Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with supe...
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| Veröffentlicht in: | Nano letters 2015-04, Vol.15 (4), p.2220-2228 |
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| creator | Morimatsu, Masatoshi Mekhdjian, Armen H Chang, Alice C Tan, Steven J Dunn, Alexander R |
| description | Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with |
| doi_str_mv | 10.1021/nl5047335 |
| format | Article |
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How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with <100 nm spatial resolution. We find that αvβ3 integrin selectively localizes to high force regions. Paxillin, which is not generally considered to play a direct role in force transmission, shows a higher degree of spatial correlation with force than vinculin, talin, or α-actinin, proteins with hypothesized roles as force transducers. 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How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with <100 nm spatial resolution. We find that αvβ3 integrin selectively localizes to high force regions. Paxillin, which is not generally considered to play a direct role in force transmission, shows a higher degree of spatial correlation with force than vinculin, talin, or α-actinin, proteins with hypothesized roles as force transducers. These observations suggest that αvβ3 integrin and paxillin may play important roles in mechanotransduction.</description><subject>Adhesion</subject><subject>Adhesiveness</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Fluorescence Resonance Energy Transfer - methods</subject><subject>Focal Adhesions - physiology</subject><subject>Humans</subject><subject>Image Enhancement - methods</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Molecular Imaging - methods</subject><subject>Molecular structure</subject><subject>Nanostructure</subject><subject>Proteins</subject><subject>Sensors</subject><subject>Stress, Mechanical</subject><subject>Tensile Strength - physiology</subject><subject>Traction</subject><subject>Transducers</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVJaJy0h_6BoksgOWwirT73UjAhqQMOgeL20oMYK1qvzHrlSNqE5Nd3WzsmhUAuM8PMw8vMvAh9oeSMkpKed60gXDEmPqARFYwUsqrKvV2t-QE6TGlJCKmYIB_RQSkUI5TTEfr9y6ceWv_suwXOjcPXXXbRh4jH0TY-O5v76HCo8VWw0OLxXeOSD13Cjz43eOIXTfHDpdD2eejiWQT7r7iBdfqE9mtok_u8zUfo59Xl7GJSTG-_X1-MpwVwpnMhra0qLhg4pSloRRUDJkUpVW0dEZpDBTAfglIVaELmda0FtTWrJZtrzdkR-rbRXffzlbuzrssRWrOOfgXxyQTw5v9J5xuzCA9GVCVXUgwCJ1uBGO57l7JZ-WRd20LnQp8MVbIkkpeMvI9KVQ5f1kIO6OkGtTGkFF2924gS89c3s_NtYL--PmFHvhg1AMcbAGwyy9DHbvjoG0J_AAj-n24</recordid><startdate>20150408</startdate><enddate>20150408</enddate><creator>Morimatsu, Masatoshi</creator><creator>Mekhdjian, Armen H</creator><creator>Chang, Alice C</creator><creator>Tan, Steven J</creator><creator>Dunn, Alexander R</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>20150408</creationdate><title>Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps</title><author>Morimatsu, Masatoshi ; Mekhdjian, Armen H ; Chang, Alice C ; Tan, Steven J ; Dunn, Alexander R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a438t-6cc99453ae781a87173a365267fce0584a9aaba9a779a800bff851cf3f63b8843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adhesion</topic><topic>Adhesiveness</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Fluorescence Resonance Energy Transfer - methods</topic><topic>Focal Adhesions - physiology</topic><topic>Humans</topic><topic>Image Enhancement - methods</topic><topic>Mechanotransduction, Cellular - physiology</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Molecular Imaging - methods</topic><topic>Molecular structure</topic><topic>Nanostructure</topic><topic>Proteins</topic><topic>Sensors</topic><topic>Stress, Mechanical</topic><topic>Tensile Strength - physiology</topic><topic>Traction</topic><topic>Transducers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morimatsu, Masatoshi</creatorcontrib><creatorcontrib>Mekhdjian, Armen H</creatorcontrib><creatorcontrib>Chang, Alice C</creatorcontrib><creatorcontrib>Tan, Steven J</creatorcontrib><creatorcontrib>Dunn, Alexander R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morimatsu, Masatoshi</au><au>Mekhdjian, Armen H</au><au>Chang, Alice C</au><au>Tan, Steven J</au><au>Dunn, Alexander R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2015-04-08</date><risdate>2015</risdate><volume>15</volume><issue>4</issue><spage>2220</spage><epage>2228</epage><pages>2220-2228</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with <100 nm spatial resolution. We find that αvβ3 integrin selectively localizes to high force regions. Paxillin, which is not generally considered to play a direct role in force transmission, shows a higher degree of spatial correlation with force than vinculin, talin, or α-actinin, proteins with hypothesized roles as force transducers. These observations suggest that αvβ3 integrin and paxillin may play important roles in mechanotransduction.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25730141</pmid><doi>10.1021/nl5047335</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nano letters, 2015-04, Vol.15 (4), p.2220-2228 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Adhesion Adhesiveness Animals Cells, Cultured Cytoskeletal Proteins - metabolism Fluorescence Resonance Energy Transfer - methods Focal Adhesions - physiology Humans Image Enhancement - methods Mechanotransduction, Cellular - physiology Membrane Proteins - metabolism Mice Microscopy Microscopy, Fluorescence - methods Molecular Imaging - methods Molecular structure Nanostructure Proteins Sensors Stress, Mechanical Tensile Strength - physiology Traction Transducers |
| title | Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps |
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