Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps

Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with supe...

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Veröffentlicht in:	Nano letters 2015-04, Vol.15 (4), p.2220-2228
Hauptverfasser:	Morimatsu, Masatoshi, Mekhdjian, Armen H, Chang, Alice C, Tan, Steven J, Dunn, Alexander R
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesion Adhesiveness Animals Cells, Cultured Cytoskeletal Proteins - metabolism Fluorescence Resonance Energy Transfer - methods Focal Adhesions - physiology Humans Image Enhancement - methods Mechanotransduction, Cellular - physiology Membrane Proteins - metabolism Mice Microscopy Microscopy, Fluorescence - methods Molecular Imaging - methods Molecular structure Nanostructure Proteins Sensors Stress, Mechanical Tensile Strength - physiology Traction Transducers
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Zusammenfassung:	Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with
ISSN:	1530-6984 1530-6992
DOI:	10.1021/nl5047335