Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells

Imaging the actin cytoskeleton in cells uses a wide range of approaches. Typically, a fluorescent derivative of the small cyclic peptide phalloidin is used to image F-actin in fixed cells. Lifeact and F-tractin are popular for imaging the cytoskeleton in live cells. Here we characterised novel affin...

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Veröffentlicht in:Scientific reports 2018-04, Vol.8 (1), p.6572-15, Article 6572
Hauptverfasser: Lopata, Anna, Hughes, Ruth, Tiede, Christian, Heissler, Sarah M., Sellers, James R., Knight, Peter J., Tomlinson, Darren, Peckham, Michelle
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Sprache:eng
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