Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis
Objective: Immunohistochemical evaluation of the degree of expression of p16 and p27 in oral epithelial dysplasia and different histological grades oral squamous carcinoma. Materials and Methods: The study consisted of 5 cases of oral squamous cell carcinoma (OSCC), 5 cases of low-risk potentially m...
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| Veröffentlicht in: | Journal of oral and maxillofacial pathology : JOMFP 2018-01, Vol.22 (1), p.59-64 |
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| container_title | Journal of oral and maxillofacial pathology : JOMFP |
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| creator | Thambiah, Lalita Bindushree, R Anjum, Afshan Pugazhendi, Satish Babu, Lokesh Nair, Renju |
| description | Objective: Immunohistochemical evaluation of the degree of expression of p16 and p27 in oral epithelial dysplasia and different histological grades oral squamous carcinoma.
Materials and Methods: The study consisted of 5 cases of oral squamous cell carcinoma (OSCC), 5 cases of low-risk potentially malignant disorders (PMDs), 5 cases of high-risk PMDs and 5 cases of normal epithelium. Five micrometer thickness sections on a positively charged slide were subjected to immunohistochemical staining for the localization of p16 and p27. The expression of p16 and p27 was assessed in 10 random high-power fields (×40). Staining intensity was graded, and the data were subjected to statistical analysis.
Results and Conclusion: OSCC and high-grade PMDs showed decreased intensity for both p16and P27. In our study, we concluded that p16 and p27 could be used as a diagnostic marker for predicting carcinogenesis in epithelial dysplasia. |
| doi_str_mv | 10.4103/jomfp.JOMFP_92_17 |
| format | Article |
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Materials and Methods: The study consisted of 5 cases of oral squamous cell carcinoma (OSCC), 5 cases of low-risk potentially malignant disorders (PMDs), 5 cases of high-risk PMDs and 5 cases of normal epithelium. Five micrometer thickness sections on a positively charged slide were subjected to immunohistochemical staining for the localization of p16 and p27. The expression of p16 and p27 was assessed in 10 random high-power fields (×40). Staining intensity was graded, and the data were subjected to statistical analysis.
Results and Conclusion: OSCC and high-grade PMDs showed decreased intensity for both p16and P27. In our study, we concluded that p16 and p27 could be used as a diagnostic marker for predicting carcinogenesis in epithelial dysplasia.</description><identifier>ISSN: 0973-029X</identifier><identifier>EISSN: 1998-393X</identifier><identifier>DOI: 10.4103/jomfp.JOMFP_92_17</identifier><identifier>PMID: 29731558</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Carcinogenesis ; Cell cycle ; Cell division ; Cyclin-dependent kinases ; Development and progression ; Diagnosis ; Dysplasia ; Epithelium ; Gene expression ; Immunohistochemistry ; Kinases ; Localization ; Maxillofacial surgery ; Multivariate analysis ; Oral cancer ; Oral squamous cell carcinoma ; Original ; Pathology ; Proteins ; Squamous cell carcinoma ; Statistical analysis ; Surgery ; Tumors</subject><ispartof>Journal of oral and maxillofacial pathology : JOMFP, 2018-01, Vol.22 (1), p.59-64</ispartof><rights>COPYRIGHT 2018 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt. Ltd. Jan/Apr 2018</rights><rights>Copyright: © 2018 Journal of Oral and Maxillofacial Pathology 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425i-80bce598c4033c55621a53f012e66682edf80d886feab276f34c2441ae5084073</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917543/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917543/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27458,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29731558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thambiah, Lalita</creatorcontrib><creatorcontrib>Bindushree, R</creatorcontrib><creatorcontrib>Anjum, Afshan</creatorcontrib><creatorcontrib>Pugazhendi, Satish</creatorcontrib><creatorcontrib>Babu, Lokesh</creatorcontrib><creatorcontrib>Nair, Renju</creatorcontrib><title>Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis</title><title>Journal of oral and maxillofacial pathology : JOMFP</title><addtitle>J Oral Maxillofac Pathol</addtitle><description>Objective: Immunohistochemical evaluation of the degree of expression of p16 and p27 in oral epithelial dysplasia and different histological grades oral squamous carcinoma.
Materials and Methods: The study consisted of 5 cases of oral squamous cell carcinoma (OSCC), 5 cases of low-risk potentially malignant disorders (PMDs), 5 cases of high-risk PMDs and 5 cases of normal epithelium. Five micrometer thickness sections on a positively charged slide were subjected to immunohistochemical staining for the localization of p16 and p27. The expression of p16 and p27 was assessed in 10 random high-power fields (×40). Staining intensity was graded, and the data were subjected to statistical analysis.
Results and Conclusion: OSCC and high-grade PMDs showed decreased intensity for both p16and P27. In our study, we concluded that p16 and p27 could be used as a diagnostic marker for predicting carcinogenesis in epithelial dysplasia.</description><subject>Carcinogenesis</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Cyclin-dependent kinases</subject><subject>Development and progression</subject><subject>Diagnosis</subject><subject>Dysplasia</subject><subject>Epithelium</subject><subject>Gene expression</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Localization</subject><subject>Maxillofacial surgery</subject><subject>Multivariate analysis</subject><subject>Oral cancer</subject><subject>Oral squamous cell carcinoma</subject><subject>Original</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Squamous cell carcinoma</subject><subject>Statistical analysis</subject><subject>Surgery</subject><subject>Tumors</subject><issn>0973-029X</issn><issn>1998-393X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1ks1u1DAUhSMEokPhAdggS6wz-CdOHBZI06qloKKyAKk7y-Ncp55J7NROOvQpeOWamU5pJZAXtny_c3RsnSx7S_C8IJh9WPneDPOvF99Ov8uaSlI9y2akrkXOanb5PJvhumI5pvXlQfYqxhXGXBScvswOaBoQzsUs-31yo7pJjda1aLwCBL-GADFa75A3aCAlUq5BA62QTTdBdQgGm8DOpmNzG4dORaviltqO4_Wkej9FpKHrkFZBW-d79REtUGNV63wcrUa9CmsIyPiwR1pwEG18nb0wqovw5n4_zH6envw4PsvPLz5_OV6c57qg3OYCLzXwWugCM6Y5LylRnBlMKJRlKSg0RuBGiNKAWtKqNKzQtCiIAo5FgSt2mH3a-Q7TsodGgxtTejkEm6LdSq-sfDpx9kq2_kbymlS8YMng_b1B8NcTxFGu_BRcyiwppqUoCabFX6pVHUjrjE9murdRywVnnBJSE5Go-T-otBrorfYOjE33TwRkJ9DBxxjAPAQnWP6phtxWQz6qRtK8e_ziB8W-Cwk42gEb340Q4rqbNhBkYtfOb_7vnL5E7lvE7gAwEM-J</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Thambiah, Lalita</creator><creator>Bindushree, R</creator><creator>Anjum, Afshan</creator><creator>Pugazhendi, Satish</creator><creator>Babu, Lokesh</creator><creator>Nair, Renju</creator><general>Wolters Kluwer India Pvt. 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Bindushree, R ; Anjum, Afshan ; Pugazhendi, Satish ; Babu, Lokesh ; Nair, Renju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425i-80bce598c4033c55621a53f012e66682edf80d886feab276f34c2441ae5084073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Carcinogenesis</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Cyclin-dependent kinases</topic><topic>Development and progression</topic><topic>Diagnosis</topic><topic>Dysplasia</topic><topic>Epithelium</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Localization</topic><topic>Maxillofacial surgery</topic><topic>Multivariate analysis</topic><topic>Oral cancer</topic><topic>Oral squamous cell carcinoma</topic><topic>Original</topic><topic>Pathology</topic><topic>Proteins</topic><topic>Squamous cell carcinoma</topic><topic>Statistical analysis</topic><topic>Surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thambiah, Lalita</creatorcontrib><creatorcontrib>Bindushree, R</creatorcontrib><creatorcontrib>Anjum, Afshan</creatorcontrib><creatorcontrib>Pugazhendi, Satish</creatorcontrib><creatorcontrib>Babu, Lokesh</creatorcontrib><creatorcontrib>Nair, Renju</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of oral and maxillofacial pathology : JOMFP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thambiah, Lalita</au><au>Bindushree, R</au><au>Anjum, Afshan</au><au>Pugazhendi, Satish</au><au>Babu, Lokesh</au><au>Nair, Renju</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis</atitle><jtitle>Journal of oral and maxillofacial pathology : JOMFP</jtitle><addtitle>J Oral Maxillofac Pathol</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>22</volume><issue>1</issue><spage>59</spage><epage>64</epage><pages>59-64</pages><issn>0973-029X</issn><eissn>1998-393X</eissn><abstract>Objective: Immunohistochemical evaluation of the degree of expression of p16 and p27 in oral epithelial dysplasia and different histological grades oral squamous carcinoma.
Materials and Methods: The study consisted of 5 cases of oral squamous cell carcinoma (OSCC), 5 cases of low-risk potentially malignant disorders (PMDs), 5 cases of high-risk PMDs and 5 cases of normal epithelium. Five micrometer thickness sections on a positively charged slide were subjected to immunohistochemical staining for the localization of p16 and p27. The expression of p16 and p27 was assessed in 10 random high-power fields (×40). Staining intensity was graded, and the data were subjected to statistical analysis.
Results and Conclusion: OSCC and high-grade PMDs showed decreased intensity for both p16and P27. In our study, we concluded that p16 and p27 could be used as a diagnostic marker for predicting carcinogenesis in epithelial dysplasia.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>29731558</pmid><doi>10.4103/jomfp.JOMFP_92_17</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of oral and maxillofacial pathology : JOMFP, 2018-01, Vol.22 (1), p.59-64 |
| issn | 0973-029X 1998-393X |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Medknow Open Access Journals; PubMed Central |
| subjects | Carcinogenesis Cell cycle Cell division Cyclin-dependent kinases Development and progression Diagnosis Dysplasia Epithelium Gene expression Immunohistochemistry Kinases Localization Maxillofacial surgery Multivariate analysis Oral cancer Oral squamous cell carcinoma Original Pathology Proteins Squamous cell carcinoma Statistical analysis Surgery Tumors |
| title | Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis |
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