Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis

Objective: Immunohistochemical evaluation of the degree of expression of p16 and p27 in oral epithelial dysplasia and different histological grades oral squamous carcinoma. Materials and Methods: The study consisted of 5 cases of oral squamous cell carcinoma (OSCC), 5 cases of low-risk potentially malignant disorders (PMDs), 5 cases of high-risk PMDs and 5 cases of normal epithelium. Five micrometer thickness sections on a positively charged slide were subjected to immunohistochemical staining for the localization of p16 and p27. The expression of p16 and p27 was assessed in 10 random high-power fields (×40). Staining intensity was graded, and the data were subjected to statistical analysis. Results and Conclusion: OSCC and high-grade PMDs showed decreased intensity for both p16and P27. In our study, we concluded that p16 and p27 could be used as a diagnostic marker for predicting carcinogenesis in epithelial dysplasia.