Production and delivery of Helicobacter pylori NapA in Lactococcus lactis and its protective efficacy and immune modulatory activity
Helicobacter pylori neutrophil-activating protein A subunit (NapA) has been identified as a virulence factor, a protective antigen and a potent immunomodulator. NapA shows unique application potentials for anti- H. pylori vaccines and treatment strategies of certain allergic diseases and carcinomas....
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Veröffentlicht in: | Scientific reports 2018-04, Vol.8 (1), p.6435-12, Article 6435 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Helicobacter pylori
neutrophil-activating protein A subunit (NapA) has been identified as a virulence factor, a protective antigen and a potent immunomodulator. NapA shows unique application potentials for anti-
H. pylori
vaccines and treatment strategies of certain allergic diseases and carcinomas. However, appropriate production and utilization modes of NapA still remain uncertain to date. This work has established a novel efficient production and utilization mode of NapA by using
L. lactis
as an expression host and delivery vector, and demonstrated immune protective efficacy and immune modulatory activity of the engineered
L. lactis
by oral vaccination of mice. It was observed for the first time that
H. pylori
NapA promotes both polarized Th17 and Th1 responses, which may greatly affect the clinical application of NapA. This report offers a promising anti-
H. pylori
oral vaccine candidate and a potent mucosal immune modulatory agent. Meanwhile, it uncovers a way to produce and deliver the oral vaccine and immunomodulator by fermentation of food like milk, which might have striking effects on control of
H. pylori
infection, gastrointestinal cancers, and Th2 bias allergic diseases, including many food allergies. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-018-24879-x |