Oxidation-induced generation of a mild electrophile for proximity-enhanced protein-protein crosslinking

We report a strategy to introduce a reactive electrophile into proteins through the conversion of a chemically inert group into a bioreactive group in response to an inducer molecule. This strategy was demonstrated by oxidation-induced and proximity-enhanced protein-protein crosslinking in the prese...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2018-04, Vol.54 (33), p.4172-4175
Hauptverfasser:	Shang, X, Chen, Y, Wang, N, Niu, W, Guo, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalysis chemical reactions Cross-Linking Reagents - chemical synthesis Cross-Linking Reagents - chemistry Crosslinking Dansyl Compounds - chemistry Escherichia coli Ethylenediamines - chemistry Fluorescent Dyes - chemistry Glutathione Transferase - chemistry Glutathione Transferase - genetics Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Hydrogen Peroxide - chemistry Lewis acids Lewis bases Lysine - analogs & derivatives Lysine - chemical synthesis Lysine - chemistry Lysine - genetics Methanosarcina barkeri Molecular Probes - chemistry Molecular Probes - genetics Mutation Myoglobin - chemistry Myoglobin - genetics Oxidation Oxidation-Reduction Proteins Proteins - chemistry Proteins - genetics Sulfoxides - chemistry Tungsten Compounds - chemistry Vinyl Compounds - chemical synthesis Vinyl Compounds - chemistry
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Beschreibung
Zusammenfassung:	We report a strategy to introduce a reactive electrophile into proteins through the conversion of a chemically inert group into a bioreactive group in response to an inducer molecule. This strategy was demonstrated by oxidation-induced and proximity-enhanced protein-protein crosslinking in the presence of a large excess of free nucleophile.
ISSN:	1359-7345 1364-548X 1364-548X
DOI:	10.1039/c8cc01639a