Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection
Purpose The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI). Methods All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispers...
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| Veröffentlicht in: | Reproductive medicine and biology 2014-04, Vol.13 (2), p.87-94 |
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| creator | Sivanarayana, T. Ravi Krishna, Ch Jaya Prakash, G. Krishna, K. M. Madan, K. Sudhakar, G. Rama Raju, G. A. |
| description | Purpose
The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI).
Methods
All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispersion (SCD) method. A total of 237 patients were in the DNA fragmentation normal group (sDFI ≤ 30 %), and 140 patients were in the DNA fragmentation abnormal group (sDFI ≥ 30 %). The relationship of sDFI with the outcome of ICSI was analyzed.
Results
A significant difference in semen parameters was observed between the DNA fragmentation normal and abnormal groups [count, motility and morphology (
p
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| doi_str_mv | 10.1007/s12522-013-0168-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5906886</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3066202173</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4578-3582b60d990961ff4ffb1376492c6ff3bdfcdc4bb52f3e4c5e0c965cbe35765b3</originalsourceid><addsrcrecordid>eNqFkctu1DAUhiMEoqXwAGyQJTZlEfAltmMWlcqUm1RAorC2bMeeepTYg52hypvwuDjNUC4CsbB85PP9v8_RX1UPEXyKIOTPMsIU4xoiUg5ra36rOkRNw2tIeXv7uqZ1KdFBdS_nDYSIiwbfrQ6wYEIgSg6rbxdbmwZw9v4UuKTWgw2jGn0MQOWsJqAnkK8Bc5niUDoBdH5-yTNzfLE6e_IcmJiS7ReZtuOVteFvhqEDcTeaOFgQHfBhTMpMY9z2Kg_e7P_xYWPNzN-v7jjVZ_tgfx9Vn1-9_LR6U59_eP12dXpem6ZsVhPaYs1gJwQUDDnXOKcR4awR2DDniO6c6UyjNcWO2MZQC41g1GhLKGdUk6PqZPHd7vRgO2PnuXq5TX5QaZJRefl7J_hLuY5fJRWQtS0rBsd7gxS_7Gwe5eCzsX2vgo27LDEkmIiWClrQx3-gm7hLoawnCWQMQ4w4KRRaKJNizsm6m2EQlHPucsldltzlnLvkRfPo1y1uFD-CLgBfgCvf2-n_jvLjuxd4LosSL8pcRGFt08-h_z3Pd2ggzIo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3066202173</pqid></control><display><type>article</type><title>Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection</title><source>Wiley Online Library Open Access</source><creator>Sivanarayana, T. ; Ravi Krishna, Ch ; Jaya Prakash, G. ; Krishna, K. M. ; Madan, K. ; Sudhakar, G. ; Rama Raju, G. A.</creator><creatorcontrib>Sivanarayana, T. ; Ravi Krishna, Ch ; Jaya Prakash, G. ; Krishna, K. M. ; Madan, K. ; Sudhakar, G. ; Rama Raju, G. A.</creatorcontrib><description>Purpose
The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI).
Methods
All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispersion (SCD) method. A total of 237 patients were in the DNA fragmentation normal group (sDFI ≤ 30 %), and 140 patients were in the DNA fragmentation abnormal group (sDFI ≥ 30 %). The relationship of sDFI with the outcome of ICSI was analyzed.
Results
A significant difference in semen parameters was observed between the DNA fragmentation normal and abnormal groups [count, motility and morphology (
p
< 0.05)]. However, no significant difference was seen between the number of oocytes retrieved and fertilization rates between the two groups, whereas the number of embryos progressed to day 3 and the blastocyst formation rate in the remaining embryos after transfer were significantly more in the DNA fragmentation normal group (
p
< 0.05). A significant negative correlation was noted between DFI values of more than 30 % and number of pregnancies and deliveries (
p
< 0.05). A higher DFI was also associated with increased abortion rates.
Conclusions
In the present study, sperm with DNA fragmentation showed a negative correlation with semen parameters. Further, sperm with damaged DNA have potential adverse effects on embryo progression, clinical pregnancy rate, and ongoing pregnancies.</description><identifier>ISSN: 1445-5781</identifier><identifier>EISSN: 1447-0578</identifier><identifier>DOI: 10.1007/s12522-013-0168-7</identifier><identifier>PMID: 29699153</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Abortion rates ; Cell cycle ; Chromatin ; Clinical pregnancy ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA fragmentation ; DNA repair ; Embryo formation rates ; Embryos ; Fertility ; Fertilization ; Gynecology ; In vitro fertilization ; Infertility ; Intracytoplasmic sperm injection ; Medicine ; Medicine & Public Health ; Morphology ; Oocytes ; Original ; Original Article ; Pregnancy ; Reproductive Medicine ; SCD ; Semen ; Sperm ; Urology</subject><ispartof>Reproductive medicine and biology, 2014-04, Vol.13 (2), p.87-94</ispartof><rights>Japan Society for Reproductive Medicine 2013</rights><rights>The Japan Society for Reproductive Medicine</rights><rights>2014. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4578-3582b60d990961ff4ffb1376492c6ff3bdfcdc4bb52f3e4c5e0c965cbe35765b3</citedby><cites>FETCH-LOGICAL-c4578-3582b60d990961ff4ffb1376492c6ff3bdfcdc4bb52f3e4c5e0c965cbe35765b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906886/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906886/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1007%2Fs12522-013-0168-7$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29699153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sivanarayana, T.</creatorcontrib><creatorcontrib>Ravi Krishna, Ch</creatorcontrib><creatorcontrib>Jaya Prakash, G.</creatorcontrib><creatorcontrib>Krishna, K. M.</creatorcontrib><creatorcontrib>Madan, K.</creatorcontrib><creatorcontrib>Sudhakar, G.</creatorcontrib><creatorcontrib>Rama Raju, G. A.</creatorcontrib><title>Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection</title><title>Reproductive medicine and biology</title><addtitle>Reprod Med Biol</addtitle><addtitle>Reprod Med Biol</addtitle><description>Purpose
The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI).
Methods
All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispersion (SCD) method. A total of 237 patients were in the DNA fragmentation normal group (sDFI ≤ 30 %), and 140 patients were in the DNA fragmentation abnormal group (sDFI ≥ 30 %). The relationship of sDFI with the outcome of ICSI was analyzed.
Results
A significant difference in semen parameters was observed between the DNA fragmentation normal and abnormal groups [count, motility and morphology (
p
< 0.05)]. However, no significant difference was seen between the number of oocytes retrieved and fertilization rates between the two groups, whereas the number of embryos progressed to day 3 and the blastocyst formation rate in the remaining embryos after transfer were significantly more in the DNA fragmentation normal group (
p
< 0.05). A significant negative correlation was noted between DFI values of more than 30 % and number of pregnancies and deliveries (
p
< 0.05). A higher DFI was also associated with increased abortion rates.
Conclusions
In the present study, sperm with DNA fragmentation showed a negative correlation with semen parameters. Further, sperm with damaged DNA have potential adverse effects on embryo progression, clinical pregnancy rate, and ongoing pregnancies.</description><subject>Abortion rates</subject><subject>Cell cycle</subject><subject>Chromatin</subject><subject>Clinical pregnancy</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA fragmentation</subject><subject>DNA repair</subject><subject>Embryo formation rates</subject><subject>Embryos</subject><subject>Fertility</subject><subject>Fertilization</subject><subject>Gynecology</subject><subject>In vitro fertilization</subject><subject>Infertility</subject><subject>Intracytoplasmic sperm injection</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Morphology</subject><subject>Oocytes</subject><subject>Original</subject><subject>Original Article</subject><subject>Pregnancy</subject><subject>Reproductive Medicine</subject><subject>SCD</subject><subject>Semen</subject><subject>Sperm</subject><subject>Urology</subject><issn>1445-5781</issn><issn>1447-0578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqFkctu1DAUhiMEoqXwAGyQJTZlEfAltmMWlcqUm1RAorC2bMeeepTYg52hypvwuDjNUC4CsbB85PP9v8_RX1UPEXyKIOTPMsIU4xoiUg5ra36rOkRNw2tIeXv7uqZ1KdFBdS_nDYSIiwbfrQ6wYEIgSg6rbxdbmwZw9v4UuKTWgw2jGn0MQOWsJqAnkK8Bc5niUDoBdH5-yTNzfLE6e_IcmJiS7ReZtuOVteFvhqEDcTeaOFgQHfBhTMpMY9z2Kg_e7P_xYWPNzN-v7jjVZ_tgfx9Vn1-9_LR6U59_eP12dXpem6ZsVhPaYs1gJwQUDDnXOKcR4awR2DDniO6c6UyjNcWO2MZQC41g1GhLKGdUk6PqZPHd7vRgO2PnuXq5TX5QaZJRefl7J_hLuY5fJRWQtS0rBsd7gxS_7Gwe5eCzsX2vgo27LDEkmIiWClrQx3-gm7hLoawnCWQMQ4w4KRRaKJNizsm6m2EQlHPucsldltzlnLvkRfPo1y1uFD-CLgBfgCvf2-n_jvLjuxd4LosSL8pcRGFt08-h_z3Pd2ggzIo</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Sivanarayana, T.</creator><creator>Ravi Krishna, Ch</creator><creator>Jaya Prakash, G.</creator><creator>Krishna, K. M.</creator><creator>Madan, K.</creator><creator>Sudhakar, G.</creator><creator>Rama Raju, G. A.</creator><general>Springer Japan</general><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201404</creationdate><title>Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection</title><author>Sivanarayana, T. ; Ravi Krishna, Ch ; Jaya Prakash, G. ; Krishna, K. M. ; Madan, K. ; Sudhakar, G. ; Rama Raju, G. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4578-3582b60d990961ff4ffb1376492c6ff3bdfcdc4bb52f3e4c5e0c965cbe35765b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abortion rates</topic><topic>Cell cycle</topic><topic>Chromatin</topic><topic>Clinical pregnancy</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA fragmentation</topic><topic>DNA repair</topic><topic>Embryo formation rates</topic><topic>Embryos</topic><topic>Fertility</topic><topic>Fertilization</topic><topic>Gynecology</topic><topic>In vitro fertilization</topic><topic>Infertility</topic><topic>Intracytoplasmic sperm injection</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Morphology</topic><topic>Oocytes</topic><topic>Original</topic><topic>Original Article</topic><topic>Pregnancy</topic><topic>Reproductive Medicine</topic><topic>SCD</topic><topic>Semen</topic><topic>Sperm</topic><topic>Urology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sivanarayana, T.</creatorcontrib><creatorcontrib>Ravi Krishna, Ch</creatorcontrib><creatorcontrib>Jaya Prakash, G.</creatorcontrib><creatorcontrib>Krishna, K. M.</creatorcontrib><creatorcontrib>Madan, K.</creatorcontrib><creatorcontrib>Sudhakar, G.</creatorcontrib><creatorcontrib>Rama Raju, G. A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Reproductive medicine and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Sivanarayana, T.</au><au>Ravi Krishna, Ch</au><au>Jaya Prakash, G.</au><au>Krishna, K. M.</au><au>Madan, K.</au><au>Sudhakar, G.</au><au>Rama Raju, G. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection</atitle><jtitle>Reproductive medicine and biology</jtitle><stitle>Reprod Med Biol</stitle><addtitle>Reprod Med Biol</addtitle><date>2014-04</date><risdate>2014</risdate><volume>13</volume><issue>2</issue><spage>87</spage><epage>94</epage><pages>87-94</pages><issn>1445-5781</issn><eissn>1447-0578</eissn><abstract>Purpose
The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI).
Methods
All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispersion (SCD) method. A total of 237 patients were in the DNA fragmentation normal group (sDFI ≤ 30 %), and 140 patients were in the DNA fragmentation abnormal group (sDFI ≥ 30 %). The relationship of sDFI with the outcome of ICSI was analyzed.
Results
A significant difference in semen parameters was observed between the DNA fragmentation normal and abnormal groups [count, motility and morphology (
p
< 0.05)]. However, no significant difference was seen between the number of oocytes retrieved and fertilization rates between the two groups, whereas the number of embryos progressed to day 3 and the blastocyst formation rate in the remaining embryos after transfer were significantly more in the DNA fragmentation normal group (
p
< 0.05). A significant negative correlation was noted between DFI values of more than 30 % and number of pregnancies and deliveries (
p
< 0.05). A higher DFI was also associated with increased abortion rates.
Conclusions
In the present study, sperm with DNA fragmentation showed a negative correlation with semen parameters. Further, sperm with damaged DNA have potential adverse effects on embryo progression, clinical pregnancy rate, and ongoing pregnancies.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>29699153</pmid><doi>10.1007/s12522-013-0168-7</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Open Access |
| subjects | Abortion rates Cell cycle Chromatin Clinical pregnancy Deoxyribonucleic acid DNA DNA damage DNA fragmentation DNA repair Embryo formation rates Embryos Fertility Fertilization Gynecology In vitro fertilization Infertility Intracytoplasmic sperm injection Medicine Medicine & Public Health Morphology Oocytes Original Original Article Pregnancy Reproductive Medicine SCD Semen Sperm Urology |
| title | Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection |
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