Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection

Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection

Purpose The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI). Methods All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispers...

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Veröffentlicht in:Reproductive medicine and biology 2014-04, Vol.13 (2), p.87-94
Hauptverfasser: Sivanarayana, T., Ravi Krishna, Ch, Jaya Prakash, G., Krishna, K. M., Madan, K., Sudhakar, G., Rama Raju, G. A.
Format: Artikel
Sprache:eng
Schlagworte:
Abortion rates
Cell cycle
Chromatin
Clinical pregnancy
Deoxyribonucleic acid
DNA
DNA damage
DNA fragmentation
DNA repair
Embryo formation rates
Embryos
Fertility
Fertilization
Gynecology
In vitro fertilization
Infertility
Intracytoplasmic sperm injection
Medicine
Medicine & Public Health
Morphology
Oocytes
Original
Original Article
Pregnancy
Reproductive Medicine
SCD
Semen
Sperm
Urology
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Zusammenfassung:Purpose The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI). Methods All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispersion (SCD) method. A total of 237 patients were in the DNA fragmentation normal group (sDFI ≤ 30 %), and 140 patients were in the DNA fragmentation abnormal group (sDFI ≥ 30 %). The relationship of sDFI with the outcome of ICSI was analyzed. Results A significant difference in semen parameters was observed between the DNA fragmentation normal and abnormal groups [count, motility and morphology ( p  
ISSN:1445-5781
1447-0578
DOI:10.1007/s12522-013-0168-7