The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Purpose This study was conducted to investigate the effects of supplementing fructose in the culture medium on in vitro maturation (IVM), in vitro fertilization (IVF), and metabolism of porcine oocytes. Methods Porcine oocytes were matured in vitro in modified North Carolina State University 37 medium (NCSU-37) and then supplemented with either glucose (5.5 mM), fructose (5.5 mM), or glucose (2.75 mM) plus fructose (2.75 mM). The maturation and fertilization of oocytes, and the incorporation and oxidation of 14 C-glucose, 14 C-fructose, and 14 C-methionine in oocytes at different stages of development were examined. Results The supplementation of glucose plus fructose significantly promoted ( P