Exploring the microbial diversity in Jordanian hot springs by comparative metagenomic analysis

A culture‐independent approach was utilized in this study to reveal the microbial diversity in Jordanian hot springs represented by Ma'in and Afra hot springs. Water samples from Ma'in and Afra hot springs were collected in June 2015. The in situ temperature of water samples range was 38–5...

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Veröffentlicht in:	MicrobiologyOpen (Weinheim) 2017-12, Vol.6 (6), p.n/a
Hauptverfasser:	Hussein, Emad I., Jacob, Jacob H., Shakhatreh, Muhamad Ali K., Abd Al‐razaq, Mutaz A., Juhmani, Abdul‐salam F., Cornelison, Christopher T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Afra Archaea Archaea - classification Archaea - genetics Archaea - isolation & purification Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Biota Computer simulation Hot springs Hot Springs - chemistry Hot Springs - microbiology Hydrogen-Ion Concentration Jordan Ma'in Metagenome Metagenomics microbial diversity Microorganisms Original Research pH effects Phylogeny Relative abundance Small mammals Water analysis Water sampling Water springs
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Beschreibung
Zusammenfassung:	A culture‐independent approach was utilized in this study to reveal the microbial diversity in Jordanian hot springs represented by Ma'in and Afra hot springs. Water samples from Ma'in and Afra hot springs were collected in June 2015. The in situ temperature of water samples range was 38–59°C and the pH range was 7.4–8.4. The metagenome was extracted and analyzed using the next generation technology (bTEFAP®). A total of 314,310 sequences were parsed and 288,452 were then clustered. The sequences were predominated by bacteria (>84%) and the relative abundance of archaea in each sample was
ISSN:	2045-8827 2045-8827
DOI:	10.1002/mbo3.521