Evidence for altered levels of Immunoglobulin D in the nasal airway mucosa of patients with chronic rhinosinusitis

Abstract Background Immunoglobulin D (IgD) is an enigmatic antibody isotype best known when co-expressed with IgM on naïve B cells. However, elevated soluble IgD (sIgD) and increased IgD+ IgM- B cell populations have been described in the human upper respiratory mucosa. Objective We assessed whether...

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Veröffentlicht in:	Journal of allergy and clinical immunology 2017-06, Vol.140 (6), p.1562-1571.e5
Hauptverfasser:	Min, Jin-Young, MD, PhD, Nayak, Jayakar V., MD, PhD, Hulse, Kathryn E., PhD, Stevens, Whitney W., MD, PhD, Raju, Paul A., PhD, Huang, Julia H., MS, Suh, Lydia A., BS, Van Roey, Griet A., PhD, Norton, James E., MS, Carter, Roderick G., BS, Price, Caroline P.E., BA, Weibman, Ava R., BA, Rashan, Ali R., MD, Ghosn, Eliver E., PhD, Patel, Zara M., MD, Homma, Tetsuya, MD, PhD, Conley, David B., MD, Welch, Kevin C., MD, Shintani-Smith, Stephanie, MD, MS, Peters, Anju T., MD, Grammer, Leslie C., MD, Harris, Kathleen E., BS, Kato, Atsushi, PhD, Hwang, Peter H., MD, Kern, Robert C., MD, Herzenberg, Leonore A., PhD, Schleimer, Robert P., PhD, Tan, Bruce K., MD, MS
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology
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Zusammenfassung:	Abstract Background Immunoglobulin D (IgD) is an enigmatic antibody isotype best known when co-expressed with IgM on naïve B cells. However, elevated soluble IgD (sIgD) and increased IgD+ IgM- B cell populations have been described in the human upper respiratory mucosa. Objective We assessed whether levels of sIgD and IgD+ B cells are altered in nasal tissue from patients with chronic rhinosinusitis (CRS). We further characterized IgD+ B cell populations and explored clinical and local inflammatory factors associated with tissue sIgD levels. Methods sIgD levels were measured by ELISA in nasal tissues, nasal lavages, serum, and supernatants of dissociated nasal tissues. IgD+ cells were identified by immunofluorescence and flow cytometry. Inflammatory mediator levels in tissues were assessed by real-time PCR and multiplex immunoassay. Bacterial cultures from the middle meatus were performed. Underlying medical history and medicine use were obtained from medical records. Results sIgD levels and the number of IgD+ cells were significantly increased in uncinate tissue (UT) of CRS without nasal polyps (CRSsNP) compared to control (4-fold, P
ISSN:	0091-6749 1097-6825
DOI:	10.1016/j.jaci.2017.05.032