Treg-mediated Suppression of Inflammation Induced by DR3 Signaling is Dependent on Galectin-9

Stimulation of several TNF receptor family proteins has been shown to dampen inflammatory disease in murine models through augmenting the number and/or activity of regulatory T cells (Treg). We recently found that one molecule, 4-1BB, utilized binding to Galectin-9 to exert its immunosuppressive effects and drive expansion of CD8 + Foxp3 − Treg. We now show that ligation of another TNFR family molecule, DR3, which has previously been found to strongly expand CD4 + Foxp3 + Treg and suppress inflammation, also requires Galectin-9. We found that the extracellular region of DR3 directly binds to Galectin-9, and that Galectin-9 associates with DR3 in Treg. From studies in vitro with Galectin-9 −/− CD4 + T cells and Treg, we found that stimulatory activity induced by ligating DR3 was in part dependent on Galectin-9. In vivo, in a model of EAE we show that an agonist of DR3 suppressed disease, correlating with expansion of CD4 + Foxp3 + Treg cells, and this protective effect was lost in Galectin-9 −/− mice. Similar results were seen in an allergic lung inflammation model. Thus, we demonstrate a novel function of Galectin-9 in facilitating activity of DR3 related to Treg-mediated suppression.