Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study
Tumor necrosis factor-alpha ( -α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 ( ) mediates -α activity and polymorphism in could lead to gene dysfunction and male infertility. The aim of this study is t...
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Veröffentlicht in: | International journal of reproductive biomedicine (Yazd, Iran) Iran), 2017-08, Vol.15 (8), p.521-526 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Tumor necrosis factor-alpha (
-α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 (
) mediates
-α activity and polymorphism in
could lead to gene dysfunction and male infertility.
The aim of this study is to determine the association of
36 A/G polymorphism with the idiopathic azoospermia in Iranian population.
This case-control study included 108 azoospermic and 119 fertile men. This research investigated the frequency of
36 A/G polymorphism in cases who were idiopathic azoospermic men referred to Yazd Research and Clinical Center for Infertility, Iran in comparison with controls. polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the polymorphism in both case and control groups. PCR fragments were digested by Mspa1I enzyme and products were appeared by gel electrophoresis. The abundance of A→G was calculated in the azoospermic and healthy men.
According to the present study, GG and AG genotypes frequency in the azoospermic men group were higher than the control group (OR= 2.298 (1.248-4.229), p=0.007), (OR=1.47 (0.869-2.498, p=0.149). Our findings also showed that G allele frequency in azoospermic men had significant difference compared to the control group (OR=2.302 (1.580-3.355), p |
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ISSN: | 2476-4108 2476-3772 |
DOI: | 10.29252/ijrm.15.8.521 |