5-Formylcytosine Yields DNA-Protein Crosslinks in Nucleosome Core Particles

5-Formylcytosine Yields DNA-Protein Crosslinks in Nucleosome Core Particles

In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein crosslinks (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amin...

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Veröffentlicht in:Journal of the American Chemical Society 2017-07, Vol.139 (31), p.10617-10620
Hauptverfasser: Li, Fengchao, Zhang, Yingqian, Bai, Jing, Greenberg, Marc M., Xi, Zhen, Zhou, Chuanzheng
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Sprache:eng
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Zusammenfassung:In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein crosslinks (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.7b05495