Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation

The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by global Pdpn deletion, we have herein explored the ef...

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Veröffentlicht in:	Journal of cellular physiology 2017-11, Vol.232 (11), p.3006-3019
Hauptverfasser:	Staines, Katherine A., Javaheri, Behzad, Hohenstein, Peter, Fleming, Robert, Ikpegbu, Ekele, Unger, Erin, Hopkinson, Mark, Buttle, David J., Pitsillides, Andrew A., Farquharson, Colin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biocompatibility Bone remodeling Bone turnover Cell Differentiation Cell Shape Computer architecture Cortical bone dendrite Dendrites Dendritic structure Differentiation E11 Elongation Embryos Female Flox Gene Deletion Genotype Glycoproteins Homeostasis Lethality Male Mechanical loading Membrane Glycoproteins - deficiency Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Mice Mice, Knockout Original Original s osteoblasts osteocalcin osteocytes Osteocytes - metabolism Osteocytes - pathology Osteogenesis Phalloidin Phenotype Phenotyping Regulatory mechanisms (biology) Signal Transduction Staining Stiffness Tibia - diagnostic imaging Tibia - metabolism Tibia - pathology X-Ray Microtomography
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Zusammenfassung:	The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by global Pdpn deletion, we have herein explored the effect of bone‐specific Pdpn knockdown on osteocyte form and function in the post‐natal mouse. Extensive skeletal phenotyping of male and female 6‐week‐old Oc‐cre;Pdpnflox/flox (cKO) mice and their Pdpnflox/flox controls (fl/fl) has revealed that Pdpn deletion significantly compromises tibial cortical bone microarchitecture in both sexes, albeit to different extents (p
ISSN:	0021-9541 1097-4652
DOI:	10.1002/jcp.25999