Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients

Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jell...

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Veröffentlicht in:Asian Pacific journal of cancer prevention : APJCP 2017-01, Vol.18 (1), p.277-286
Hauptverfasser: Mirshamsi, Mohammad Reza, Omranipour, Ramesh, Vazirizadeh, Amir, Fakhri, Amir, Zangeneh, Fatemeh, Mohebbi, Gholam Hussain, Seyedian, Ramin, Pourahmad, Jalal
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Sprache:eng
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Zusammenfassung:Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed. Result: Our results demonstrate that fraction 2 of Cassiopea andromeda crude venom significantly (P
ISSN:1513-7368
2476-762X
DOI:10.22034/APJCP.2017.18.1.277