Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients
Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jell...
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Veröffentlicht in: | Asian Pacific journal of cancer prevention : APJCP 2017-01, Vol.18 (1), p.277-286 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom,
could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas.
Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex
G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast
tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen
species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of
caspase3 and apoptosis were then assayed. Result: Our results demonstrate that fraction 2 of Cassiopea andromeda
crude venom significantly (P |
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ISSN: | 1513-7368 2476-762X |
DOI: | 10.22034/APJCP.2017.18.1.277 |