72 residues of gal repressor fused to beta‐galactosidase repress the gal operon of E. coli

An active gene has been constructed which produces a chimera consisting of the N‐terminal domain of the gal repressor and all but the first five residues of beta‐galactosidase. Seventy two residues of gal repressor fused to beta‐galactosidase as tetrameric core are sufficient to repress the gal oper...

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Veröffentlicht in:	The EMBO journal 1983, Vol.2 (8), p.1271-1274
Hauptverfasser:	Wilcken‐Bergmann, B., Koenen, M., Griesser, H.W., Müller‐Hill, B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Artificial Gene Fusion Base Sequence beta-Galactosidase - genetics DNA, Bacterial Escherichia coli Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Molecular Sequence Data Operator Regions, Genetic Operon Repressor Proteins - genetics
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Beschreibung
Zusammenfassung:	An active gene has been constructed which produces a chimera consisting of the N‐terminal domain of the gal repressor and all but the first five residues of beta‐galactosidase. Seventy two residues of gal repressor fused to beta‐galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.
ISSN:	0261-4189 1460-2075
DOI:	10.1002/j.1460-2075.1983.tb01580.x