Monitoring focal adhesion kinase phosphorylation dynamics in live cells

Monitoring focal adhesion kinase phosphorylation dynamics in live cells

Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we...

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Veröffentlicht in:Analyst (London) 2017-07, Vol.142 (15), p.2713-2716
Hauptverfasser: Damayanti, Nur P, Buno, Kevin, Narayanan, Nagarajan, Voytik Harbin, Sherry L, Deng, Meng, Irudayaraj, Joseph M K
Format: Artikel
Sprache:eng
Schlagworte:
Biosensing Techniques
Cells, Cultured
Fluorescence
Focal Adhesion Protein-Tyrosine Kinases - chemistry
Humans
Microscopy, Confocal
Phosphorylation
Single-Cell Analysis
Tyrosine
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Zusammenfassung:Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.
ISSN:0003-2654
1364-5528
1364-5528
DOI:10.1039/c7an00471k